The aim of the present study was to synthesize a novel biopolymeric micelle based on punicic acid (PA) and polyacrylamide (PAM) for carrying chemotherapeutic drugs used in prostate cancer treatment. A polymer composite micelle was prepared by chemical conjugation between PAM and PA. The micelles were prepared by self-assembly via film casting followed by ultrasonication method. The successful production of PAMPA copolymeric micelles was confirmed using FTIR, 1H-NMR, and TEM. Then, flutamide was loaded in the designed nanomicelles and they were characterized. The cell cytotoxicity of the micelles was studied on PC3 cells of prostate cancer. The prepared nanomicelles showed the particle size of 88 nm, PDI of 0.246, zeta potential of -9 mV, drug loading efficiency of 94.5%, drug release of 85.6% until 10 hours in pH 7.4 and CMC of 74.13 μg/ml. The cell viability in blank nanocarriers was about 70% in PC3 cells at concentration of 25 μM. More significant cytotoxic effects were seen for flutamide loaded micelles at this concentration compared to the free drug. The results suggest that the PAMPA co-polymeric nanomicelles can be utilized as an effective carrier to enhance the cytotoxic effects of flutamide in prostate cancer.

中文翻译：

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用于前列腺癌 PC3 细胞中氟他胺递送的基于聚丙烯酰胺-石榴酸缀合物的胶束：合成、表征和细胞毒性研究。


本研究的目的是合成一种基于石榴酸（PA）和聚丙烯酰胺（PAM）的新型生物聚合物胶束，用于携带用于前列腺癌治疗的化疗药物。通过PAM和PA之间的化学共轭制备了聚合物复合胶束。通过流延薄膜自组装和超声处理方法制备胶束。使用 FTIR、1H-NMR 和 TEM 证实了 PAMPA 共聚物胶束的成功生产。然后，将氟他胺负载到设计的纳米胶束中并对其进行表征。在前列腺癌的 PC3 细胞上研究了胶束的细胞毒性。制备的纳米胶束粒径为88 nm，PDI为0.246，zeta电位为-9 mV，载药率为94.5%，在pH 7.4和CMC为74.13 μg/ml中10小时内药物释放率为85.6%。在浓度为 25 μM 的 PC3 细胞中，空白纳米载体中的细胞活力约为 70%。与游离药物相比，在此浓度下加载氟他胺的胶束观察到更显着的细胞毒性作用。结果表明，PAMPA 共聚物纳米胶束可以作为有效载体来增强氟他胺在前列腺癌中的细胞毒作用。