Fluconazole-resistant Candida albicans is a big scary reality. The authors assessed the antifungal effects of magnetic iron-oxide nanoparticles on fluconazole-resistant colonising isolate of C. albicans and determined the expression of ERG11 gene, protein sequence similarity and ergosterol content. C. albicans isolates were characterised and fluconazole resistance is recognised using World Health Organization's WHONET software. Susceptibility testing of magnetic iron-oxide nanoparticles against fluconazole-resistant colonising isolate of C. albicans was performed according to Clinical and Laboratory Standards Institute guidelines. The expression patterns of ERG11 and protein sequence similarity were investigated. Ergosterol quantification has been used to gauge the antifungal activity of magnetic iron-oxide nanoparticles. The findings indicated that 93% of C. albicans isolates were resistant to fluconazole. Magnetic iron-oxide nanoparticles were presented activity against fluconazole-resistant colonising isolate of C. albicans with minimum inhibitory concentration at 250-500 µg/ml. The expression level of ERG11 gene was downregulated in fluconazole-resistant colonising isolate of C. albicans. The results revealed no differences in fluconazole-resistant colonising isolate of C. albicans by comparison with ERG11 reference sequences. Moreover, significant reduction was noted in ergosterol content. The findings shed a novel light on the application of magnetic iron-oxide nanoparticles in fighting against resistant C. albicans.

中文翻译：

---

磁性氧化铁纳米颗粒对氟康唑耐药白色念珠菌定植分离株羊毛甾醇 14α-去甲基化酶 (ERG11) 表达模式的抑制作用。

耐氟康唑的白色念珠菌是一个可怕的现实。作者评估了磁性氧化铁纳米粒子对白色念珠菌耐氟康唑定植分离株的抗真菌作用，并确定了 ERG11 基因的表达、蛋白质序列相似性和麦角甾醇含量。使用世界卫生组织的 WHONET 软件对白色念珠菌分离株进行了表征，并确认了对氟康唑的耐药性。根据临床和实验室标准协会指南，对磁性氧化铁纳米颗粒对白色念珠菌的耐氟康唑定植分离物进行敏感性测试。研究了ERG11的表达模式和蛋白质序列相似性。麦角甾醇定量已被用于测量磁性氧化铁纳米颗粒的抗真菌活性。研究结果表明，93% 的白色念珠菌分离株对氟康唑具有抗药性。磁性氧化铁纳米颗粒对白色念珠菌的耐氟康唑定植分离物具有活性，最低抑制浓度为 250-500 µg/ml。ERG11 基因的表达水平在白色念珠菌的耐氟康唑定植分离物中下调。结果显示，与 ERG11 参考序列相比，白色念珠菌的耐氟康唑定植分离株没有差异。此外，注意到麦角甾醇含量显着降低。这些发现为磁性氧化铁纳米颗粒在对抗耐药性白色念珠菌中的应用提供了新的思路。磁性氧化铁纳米颗粒对白色念珠菌的耐氟康唑定植分离物具有活性，最低抑制浓度为 250-500 µg/ml。ERG11 基因的表达水平在白色念珠菌的耐氟康唑定植分离物中下调。结果显示，与 ERG11 参考序列相比，白色念珠菌的耐氟康唑定植分离株没有差异。此外，注意到麦角甾醇含量显着降低。这些发现为磁性氧化铁纳米颗粒在对抗耐药性白色念珠菌中的应用提供了新的思路。磁性氧化铁纳米颗粒对白色念珠菌的耐氟康唑定植分离物具有活性，最低抑制浓度为 250-500 µg/ml。ERG11 基因的表达水平在白色念珠菌的耐氟康唑定植分离物中下调。结果显示，与 ERG11 参考序列相比，白色念珠菌的耐氟康唑定植分离株没有差异。此外，注意到麦角甾醇含量显着降低。这些发现为磁性氧化铁纳米颗粒在对抗耐药性白色念珠菌中的应用提供了新的思路。结果显示，与 ERG11 参考序列相比，白色念珠菌的耐氟康唑定植分离株没有差异。此外，注意到麦角甾醇含量显着降低。这些发现为磁性氧化铁纳米颗粒在对抗耐药性白色念珠菌中的应用提供了新的思路。结果显示，与 ERG11 参考序列相比，白色念珠菌的耐氟康唑定植分离株没有差异。此外，注意到麦角甾醇含量显着降低。这些发现为磁性氧化铁纳米颗粒在对抗耐药性白色念珠菌中的应用提供了新的思路。