Chronic wounds are a prominent concern, accounting for $25 billion of health care costs annually. Biofilms have been implicated in delayed wound closure, but they are susceptible to developing antibiotic resistance and treatment options continue to be limited. A novel collagen-rich hydrogel derived from human extracellular matrix presents an avenue for treating chronic wounds by providing appropriate extracellular proteins for healing and promoting neovascularization. Using the hydrogel as a delivery system for localized secretion of a therapeutic dosage of antibiotics presents an attractive means of maximizing delivery while minimizing systemic side effects. We hypothesize that the hydrogel can provide controlled elution of antibiotics leading to inhibition of bacterial growth and disruption of biofilm formation. The rate of antibiotic elution from the collagen-rich hydrogel and the efficacy of biofilm disruption was assessed with Pseudomonas aeruginosa. Bacterial growth inhibition, biofilm disruption, and mammalian cell cytotoxicity were quantified using in vitro models. The antibiotic-loaded hydrogel showed sustained release of antibiotics for up to 24 h at therapeutic levels. The treatment inhibited bacterial growth and disrupted biofilm formation at multiple time points. The hydrogel was capable of accommodating various classes of antibiotics and did not result in cytotoxicity in mammalian fibroblasts or adipose stem cells. The antibiotic-loaded collagen-rich hydrogel is capable of controlled antibiotic release effective for bacteria cell death without native cell death. A human-derived hydrogel that is capable of eluting therapeutic levels of antibiotic is an exciting prospect in the field of chronic wound healing.

中文翻译：

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富含胶原蛋白的水凝胶中的局部抗生素洗脱可成功抑制细菌生长和体外生物膜形成。

慢性伤口是一个突出的问题，每年要花费250亿美元的医疗费用。生物膜与伤口闭合延迟有关，但它们易受抗生素耐药性的影响，治疗选择继续受到限制。来源于人细胞外基质的新型富含胶原蛋白的水凝胶为通过提供适当的细胞外蛋白来治愈和促进新血管形成提供了治疗慢性伤口的途径。使用水凝胶作为局部分泌治疗剂量的抗生素的传递系统提供了一种使传递最大化，同时使系统性副作用最小的有吸引力的手段。我们假设水凝胶可以提供可控的抗生素洗脱，从而抑制细菌生长和破坏生物膜的形成。铜绿假单胞菌。使用体外模型量化细菌生长抑制，生物膜破坏和哺乳动物细胞的细胞毒性。载有抗生素的水凝胶在治疗水平上显示抗生素持续释放长达24小时。该处理在多个时间点抑制细菌生长并破坏生物膜形成。水凝胶能够容纳各种类型的抗生素，并且不会在哺乳动物成纤维细胞或脂肪干细胞中产生细胞毒性。载有抗生素的富含胶原蛋白的水凝胶能够控制抗生素的释放，从而有效地杀死细菌而无天然细胞死亡。能够洗脱抗生素治疗水平的人源水凝胶在慢性伤口愈合领域是令人兴奋的前景。