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Ceratocystis fimbriata employs a unique infection strategy targeting peltate glandular trichomes of sweetpotato (Ipomoea batatas) plants.
Phytopathology ( IF 2.6 ) Pub Date : 2020-11-04 , DOI: 10.1094/phyto-05-20-0165-r
Yong Sun 1 , Mengqiu Li 1 , Yansu Wang 1 , Lianwei Li 1 , Meng Wang 1 , Xintong Li 1 , Mengke Xu 1 , Gary J Loake 2, 3 , Ming Guo 1 , Jihong Jiang 1, 2
Affiliation  

The infection processes of Ceratocystis fimbriata BMPZ13 (BMPZ13) was elucidated on vegetative tissues of sweetpotato plants employing light and scanning electron microscopy. Vegetative tissues infected with C. fimbriata BMPZ13 by either wounding or nonwounding inoculation methods developed typical disease symptoms, establishing black rot in stems and necrosis on buds, young leaves, and stems of sprouts, in addition to wilt on leaves and shoot cuttings, typical of vascular associated diseases. The runner hyphae of C. fimbriata BMPZ13 formed from germinated conidia were able to directly penetrate the epidermal cuticle for initial infection and invade sweetpotato peltate glandular trichomes, specialized secretory structures to store and secrete metabolites. A two-step biotrophic phase was observed with nonwounding inoculation on leaves and stems, featuring both intercellular and intracellular invasive hyphae, with the latter found within living cells of the leaf epidermis. Subsequent to the biotrophic phase was a necrotrophic phase displaying cell death in infected leaves and veins. Additionally, this cell death was an iron-associated ferroptosis, supporting the notion that iron is involved in the necrotrophic phase of C. fimbriata BMPZ13 infection. Significantly, we establish that C. fimbriata employs a unique infection strategy: the targeting of peltate glandular trichomes. Collectively, our findings show that C. fimbriata is a plant fungal pathogen with a hemibiotrophic infection style in sweetpotato vegetative tissues.



中文翻译:

Ceratocystis fimbriata采用一种独特的感染策略,针对甘薯(Ipomoea batatas)植物的带齿腺毛状体。

利用光镜和扫描电镜,阐明了甘薯植物营养组织上的纤毛角膜藻BMPZ13(Cf BMPZ13)的感染过程。通过伤口接种或非伤口接种方法感染了Cf BMPZ13的营养组织出现了典型的疾病症状,除了茎叶枯萎以外,还出现了茎杆黑腐病,芽,幼叶和芽茎坏死。血管相关疾病。由萌发的分生孢子形成的Cf BMPZ13的亚军菌丝能够直接穿透表皮角质层进行初始感染,并侵入甘薯盾形的腺毛(PGT),专门的分泌结构来存储和分泌代谢产物。观察到两步生物营养阶段,叶片和茎上未受伤,具有细胞间和细胞内侵入性菌丝(IH)的特征,后者在叶表皮的活细胞内发现。在生物营养阶段之后是一个坏死营养阶段,显示出受感染的叶片和静脉中的细胞死亡。另外,这种细胞死亡是与铁有关的肥大病,支持了铁参与Cf BMPZ13感染的坏死阶段的观点。重要的是,我们确定纤毛衣原体采用了独特的感染策略:针对盾状腺毛的目标。总的来说,我们的发现表明纤毛衣藻是一种植物真菌病原体,在甘薯的营养组织中具有半营养营养型感染。在生物营养阶段之后是一个坏死营养阶段,显示出受感染的叶片和静脉中的细胞死亡。另外,这种细胞死亡是与铁有关的肥大病,支持了铁参与Cf BMPZ13感染的坏死阶段的观点。重要的是,我们确定纤毛衣原体采用了独特的感染策略:针对盾状腺毛的目标。总的来说,我们的发现表明纤毛衣藻是一种植物真菌病原体,在甘薯的营养组织中具有半营养营养型感染。在生物营养阶段之后是一个坏死营养阶段,显示出受感染的叶片和静脉中的细胞死亡。另外,这种细胞死亡是与铁有关的肥大病,支持了铁参与Cf BMPZ13感染的坏死阶段的观点。重要的是,我们确定纤毛衣藻使用独特的感染策略:靶向有齿的腺毛。总的来说,我们的发现表明纤毛衣藻是一种植物真菌病原体,在甘薯的营养组织中具有半营养营养型感染。针对有齿的腺毛。总的来说,我们的发现表明纤毛衣藻是一种植物真菌病原体,在甘薯的营养组织中具有半营养营养型感染。针对有齿的腺毛。总的来说,我们的发现表明纤毛衣藻是一种植物真菌病原体,在甘薯的营养组织中具有半营养营养型感染。
更新日期:2020-12-05
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