Neutron diffraction studies of hydrogen/deuterium-exchanged hen egg-white lysozyme were performed by a joint X-ray and neutron refinement to elucidate the hydrogen/deuterium exchange behavior. Large crystals for neutron work, consisting of molecules that were exchanged before crystallization, were obtained by repeatedly adding protein solution to the crystal batch using deuterated precipitant reagent. There are differences in hydrogen/deuterium exchange behavior compared with previous crystallographic or NMR studies, which could be due to intermolecular interactions in the crystal or to different lengths of exchange period.

中文翻译：

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溶液状态对四方母鸡蛋清溶菌酶晶体氢/氘交换行为的影响

氢/氘交换的鸡蛋清溶菌酶的中子衍射研究是通过联合 X 射线和中子精修进行的，以阐明氢/氘交换行为。用于中子工作的大晶体，由在结晶前交换的分子组成，通过使用氘沉淀剂向晶体批次中重复添加蛋白质溶液而获得。与之前的晶体学或核磁共振研究相比，氢/氘交换行为存在差异，这可能是由于晶体中的分子间相互作用或不同的交换周期长度。