Portable electroantennograms (pEAG) can further our understanding of odor plume dynamics and complement laboratory-based electroantennogram tools. pEAG’s can help to address important questions such as the influence of plume structure on insect behavior, the active space of semiochemical-baited traps, and the impact of biotic and abiotic factors on this active space. Challenges associated with pEAGs include their miniaturization and sensitivity, confounding environmental odors, and processing of data. Here, we describe a pEAG built with modern engineering hardware and techniques that is portable in being both light in weight (516 g) and smaller (12 × 12 × 8 cm, volume 1152 cm³) than earlier models. It is able to incorporate insects of a range of sizes (4 to 30 mm antennal length), has wireless communication (communication range of 600 m urban, 10 km line of sight), a stand-alone power supply, and uses both antennae of the test insect. We report normalized antennal responses from Epiphyas postvittana in a dose response experiment where our pEAG compared favorably with traditional laboratory EAG equipment for this species. Dose-response comparisons between E. postvittana, Agrotis ipsilon, and Lymantria dispar dispar showed mean detection limits from a pheromone source dose of 100, 100, and 1 ng, respectively, for our pEAG. This pEAG should allow future real-time analysis of EAG responses in the field in research on how insects interact with odor plumes and the factors that influence the active space of semiochemical-baited traps.

中文翻译：

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轻型便携式电子肛门造影设备，是未来基于现场的应用化学生态学工具。

便携式心电图（pEAG）可以进一步加深我们对气味羽流动力学的理解，并补充基于实验室的心电图工具。pEAG可以帮助解决重要问题，例如羽状结构对昆虫行为的影响，用化学信息诱饵诱集的陷阱的活动空间以及生物和非生物因素对该活动空间的影响。与pEAG相关的挑战包括其小型化和敏感性，混杂的环境气味以及数据处理。在这里，我们描述了一种采用现代工程硬件和技术构建的pEAG，该便携式设备既轻巧（516 g）又小（12×12×8 cm，体积1152 cm ^3））。它能够容纳各种大小的昆虫（触角长度为4至30 mm），具有无线通信功能（城市范围内通信距离为600 m，视距为10 km），具有独立电源，并且使用以下两种天线测试昆虫。我们在剂量反应实验中报告了埃弗菲酵母（Epiphyas postvittana）的标准化触角反应，其中我们的pEAG与该物种的传统实验室EAG设备相比具有优势。E. postvittana，Agrotis ipsilon和Lymantria dispar dispar之间的剂量反应比较分别显示了我们的pEAG来自100、100和1 ng信息素源剂量的平均检测限。该pEAG应该可以用于未来在昆虫中如何与气味羽流相互作用以及影响受化学信息诱捕的诱集器的活动空间的因素进行研究的田间EAG响应的实时分析。