Pavarotti, the Drosophila MKLP1 orthologue, is a kinesin-like protein that works with Tumbleweed (MgcRacGAP) as the centralspindlin complex. This complex is essential for cytokinesis, where it helps to organize the contractile actomyosin ring at the equator of dividing cells by activating the RhoGEF Pebble. Actomyosin rings also function as the driving force during cell wound repair. We previously showed that Tumbleweed and Pebble are required for the cell wound repair process. Here, we show that Pavarotti also functions during wound repair and confirm that while Pavarotti, Tumbleweed, and Pebble are all used during this cellular repair, each has a unique localization pattern and knockdown phenotype, demonstrating centralspindlin-independent functions. Surprisingly, we find that the classically microtubule-associated Pavarotti binds directly to actin in vitro and in vivo and has a noncanonical role directly regulating actin dynamics. Finally, we demonstrate that this actin regulation by Pavarotti is not specific to cellular wound repair but is also used in normal development.

中文翻译：

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类似驱动蛋白的帕瓦罗蒂（Pavarotti）以非常规的方式调节肌动蛋白动力学

Pavarotti 是果蝇 MKLP1 直系同源物，是一种类似驱动蛋白的蛋白，与风滚草 (MgcRacGAP) 一起作为中枢纺锤蛋白复合物发挥作用。该复合物对于胞质分裂至关重要，它通过激活 RhoGEF Pebble 帮助在分裂细胞的赤道组织收缩肌动球蛋白环。肌动球蛋白环还充当细胞伤口修复过程中的驱动力。我们之前表明风滚草和卵石是细胞伤口修复过程所必需的。在这里，我们证明帕瓦罗蒂也在伤口修复过程中发挥作用，并证实虽然帕瓦罗蒂、风滚草和卵石都在这种细胞修复过程中使用，但每种都具有独特的定位模式和敲低表型，展示了中枢纺锤体独立的功能。令人惊讶的是，我们发现经典的微管相关的帕瓦罗蒂在体外和体内直接与肌动蛋白结合，并且具有直接调节肌动蛋白动力学的非典型作用。最后，我们证明帕瓦罗蒂的这种肌动蛋白调节并非特定于细胞伤口修复，而是也用于正常发育。