Phenanthrene (Phe) female rat model was established to explore the mechanism of Phe on immune impairment. The rats were randomly divided into three groups, including control (C), low (L), and high (H) groups. Phe was supplied to L and H groups at the dose of 180 and 900 mg/kg orally at first day and with the dose of 90 and 450 mg/kg by intraperitoneal injection at the last 2 days. The C group was enriched with the same volume of corn oil. The liver tissue was collected. Then, the protein and mRNA expressions of interleukin (IL)-35 and the concentration IL-17 were detected to evaluate the function of regulatory T cells (Treg cells) and T helper 17 cells (Th17 cells). In addition, IL-1β and interferon-γ (IFN-γ) were analyzed to evaluate the immune impairment. The results showed that the protein and mRNA expressions of IL-35 decreased significantly in H groups (P < 0.05). Meanwhile, there were significant increases in IL-17, IFN-γ and IL-1β in the liver of H group (P < 0.05). This study demonstrated that Phe exposure might be associated with the immune impairment via changing inflammatory mediators including IL-35 and IL-17 in female rats.

中文翻译：

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菲暴露对雌性大鼠 Treg 和 Th17 细胞相关细胞因子的影响。


建立菲（Phe）雌性大鼠模型，探讨Phe对免疫损伤的作用机制。将大鼠随机分为三组，包括对照组（C）、低组（L）和高组（H）。 L组和H组第一天口服Phe剂量为180和900 mg/kg，最后2天腹腔注射Phe剂量为90和450 mg/kg。 C组添加等体积的玉米油。收集肝组织。然后检测白细胞介素(IL)-35蛋白和mRNA的表达以及IL-17的浓度，以评估调节性T细胞(Treg细胞)和辅助性T细胞17细胞(Th17细胞)的功能。此外，分析IL-1β和干扰素-γ（IFN-γ）以评估免疫损伤。结果显示，H组IL-35蛋白及mRNA表达显着降低（ P <<0.05）。同时，H组肝脏中IL-17、IFN-γ和IL-1β显着升高（ P <<0.05）。这项研究表明，Phe 暴露可能通过改变雌性大鼠中炎症介质（包括 IL-35 和 IL-17）与免疫损伤有关。