To study the extraction technology of polysaccharides (AAP) from Chinese herbal medicine formula and its mechanism of delaying aging. First, L9(3)4 orthogonal test was used to optimize the optimal enzyme-assisted extraction parameters of polysaccharides. And the anti-aging effects was evaluated by detecting mitochondrial function, protein, DNA, adhesion molecules and cell cycle in aging rats. The optimal extraction process parameters were the cellulase concentration of 1.5%, the pH at 5, the enzyme temperature at 50°C and the extraction time of 180 min. The anti-aging results showed that AAP can effectively increase the activities of malate dehydrogenase, succinate dehydrogenase and superoxide dismutase. It also can decrease the activity of monoamine oxidase and methane dicarboxylic aldehyde levels in the brain tissue. Meanwhile, the polysaccharides enhanced telomerase activity while reduced p16 protein expression of the brain mitochondria. In addition, the polysaccharides continued to improve heart damage and significantly lessen mitochondrial DNA concentrations. For a certain period of time, it also enhanced the activity of superoxide dismutase, reduced glutathione, glutathione peroxidase and decreased protein carbonyl and methane dicarboxylic aldehyde content of kidney in D-galactose-induced aging rats. Furthermore, the polysaccharides restored the number of cells in the peripheral blood lines and BMNC through inhibiting the drop of the number of red blood cells, white blood cells, platelets in the peripheral blood and bone marrow mononuclear cell of the aging rats. At the same time, AAP accelerated G1 phase cell to enter S phase in cell cycle in aging rats. Our research suggests that the polysaccharides may be a potential anti-aging agent and can be further developed as a functional food or new drug to delay aging or treat aging-related diseases.

中文翻译：

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中草药配方多糖延缓衰老的优化及其机理。

研究中草药配方多糖（AAP）的提取工艺及其延缓衰老的机理。首先，使用L9（3）4正交试验优化多糖的最佳酶辅助提取参数。通过检测衰老大鼠的线粒体功能，蛋白质，DNA，粘附分子和细胞周期来评估其抗衰老作用。最佳提取工艺参数为纤维素酶浓度为1.5％，pH为5，酶温度为50°C和提取时间为180分钟。抗衰老结果表明，AAP能有效提高苹果酸脱氢酶，琥珀酸脱氢酶和超氧化物歧化酶的活性。它还可以降低脑组织中单胺氧化酶的活性和甲烷二羧酸醛的水平。与此同时，多糖增强端粒酶活性，同时降低脑线粒体的p16蛋白表达。此外，多糖继续改善心脏损伤并显着降低线粒体DNA浓度。在一定时期内，它还增强了D-半乳糖所致衰老大鼠肾脏中超氧化物歧化酶的活性，减少了谷胱甘肽，谷胱甘肽过氧化物酶，并降低了肾脏的蛋白质羰基和甲烷二羧酸醛含量。此外，多糖通过抑制衰老大鼠的外周血红细胞，白细胞，血小板和骨髓单核细胞数目的下降，恢复了外周血细胞和BMNC中的细胞数目。同时，AAP促进衰老大鼠细胞周期中的G1期细胞进入S期。