Benzyl butyl phthalate (BBP) is a persistent environmental pollutant. BBP exposure and the possible effects on human neural tube defects (NTDs) remain elusive. In this study, we found that the detection ratio of positive BBP and its metabolites in maternal urine was obviously higher in NTDs’ population than that in normal controls by GC-MS (P < 0.01, P < 0.05, respectively). Animal experiments showed that BBP treatment induced developmental toxicity in chick embryo by enhancing the levels of oxidative stress and cell apoptosis (P < 0.01). More interestingly, the supplement of high-dose choline (CHO, 10 ⁵ μg/mL) could partially restore the teratogenic effects of BBP by inhibiting the occurrence of oxidative stress. Our data collectively suggest that BBP exposure may disturb neural tube development by strengthening oxidative stress. CHO can partially restore the toxicity effects of BBP. This study may provide new insight for NTD prevention.

中文翻译：

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氧化应激反应与邻苯二甲酸苄基丁酯（BBP）的致畸作用有关。

邻苯二甲酸苄基丁酯（BBP）是一种持久的环境污染物。BBP暴露以及对人神经管缺陷（NTD）的可能影响仍然难以捉摸。在这项研究中，我们发现NTD人群中母体尿中BBP阳性及其代谢产物的检出率显着高于正常对照者的GC-MS检测（分别为P <  0.01，P <  0.05）。动物实验表明，BBP处理可通过提高氧化应激水平和细胞凋亡来诱导雏鸡胚胎发育发育（P <  0.01）。更有趣的是，补充大剂量胆碱（CHO，10 ⁵ μg/ mL）通过抑制氧化应激的发生可以部分恢复BBP的致畸作用。我们的数据共同表明，BBP暴露可能会通过增强氧化应激而干扰神经管的发育。CHO可以部分恢复BBP的毒性作用。这项研究可能为NTD预防提供新的见解。