We recently reported the recovery of a conjugative virulence plasmid, p15WZ-82_Vir, from a clinical Klebsiella variicola strain. In this study, we found that several new plasmid types were generated due to genetic rearrangement. Partial integration of plasmid p15WZ-82_Vir with existing plasmids such as resistance plasmids by different homologous recombination events was observable in three recipient strains. Such recombination events enable the formation of various types of mosaic plasmids simultaneously carrying virulence-encoding and antibiotic resistance-encoding genes as well as genes involved in plasmid conjugation, which promote transmission of various virulence-encoding and resistance-encoding elements among pathogens. Our data also suggest that these conjugative events may play an integral role in the development of novel mosaic plasmids, which is vital for plasmid evolution.

中文翻译：

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跟踪在传播过程中共轭毒力质粒p15WZ-82_Vir中发生的重组事件。

我们最近报道了从临床水痘克雷伯菌中回收结合性毒力质粒p15WZ- 82_Vir应变。在这项研究中，我们发现由于基因重排产生了几种新的质粒类型。在三个受体菌株中观察到质粒p15WZ-82_Vir与现有质粒如抗性质粒通过不同的同源重组事件的部分整合。这样的重组事件使得能够形成同时携带毒力编码和抗生素抗性编码基因以及参与质粒缀合的基因的各种类型的镶嵌质粒，这促进了病原体之间各种毒力编码和抗性编码元件的传递。我们的数据还表明，这些结合事件可能在新型镶嵌质粒的发展中起着不可或缺的作用，这对质粒的进化至关重要。