The objective of this study was to investigate the effects of difloxacin (DIF) and avermectin (AVM) on glutamate decarboxylase (GAD) and GABA-transaminase (GABA-T) in different tissues of crucian carp (Carassius auratus gibelio). After the treatments of DIF and AVM, the mRNA expressions of GAD and GABA-T in different tissues were detected by quantitative real-time PCR (qPCR). The results showed that the mRNA expressions of GAD₆₅, GAD₆₇, and GABA-T in the telencephalon (Tel), mesencephalon (Mes), cerebella (Cer), and medulla oblongata (Med) were downregulated significantly with the safe dose (SD, 20 mg/kg) of DIF (P < 0.05 or P < 0.01). While the expressions of GAD₆₅ and GAD₆₇ in the kidney at 12 h had strikingly upregulated to 13.81 ± 1.06** and 150.67 ± 12.85** times. Treated with the lethal dose of 50% (LD₅₀, 2840 mg/kg b. W.) of DIF, the mRNA expressions of GAD₆₅, GAD₆₇, and GABA-T in all tissues were increased significantly (P < 0.01). The results of AVM group showed that the mRNA expressions of GAD₆₅, GAD₆₇, and GABA-T both in the central and peripheral tissues were all remarkably downregulated at the safe concentration (SC, 0.0039 mg/L) and the lethal concentration of 50% (LC₅₀, 0.039 mg/L), except for the mRNA inhibitions of GAD₆₅, GAD₆₇, and GABA-T in the muscle at 2 h which sharply downregulated to 0.20 ± 0.02^ΔΔ × 10⁻², 0.57 ± 0.06^ΔΔ × 10⁻¹ and 0.44 ± 0.02^ΔΔ × 10⁻¹, respectively (P < 0.01).

本研究的目的是调查二氟沙星（DIF）和阿维菌素（AVM）上谷氨酸脱羧酶（GAD）和鲫鱼的不同组织中GABA转氨酶（GABA-T）（的影响银鲫）。DIF和AVM处理后，通过实时定量PCR（qPCR）检测GAD和GABA-T在不同组织中的mRNA表达。结果表明，安全剂量（SD）可显着下调端脑（Tel），中脑（Mes），小脑（Cer）和延髓（Med）中GAD ₆₅，GAD ₆₇和GABA-T的mRNA表达。，20 mg / kg）的DIF（P  <0.05或P  <0.01）。而GAD ₆₅和GAD的表达肾脏12小时的₆₇倍显着上调至13.81±1.06 **和150.67±12.85 **次。为50％（LD的致死剂量处理₅₀，2840毫克/公斤湾W.）DIF的，GAD的mRNA表达₆₅，GAD ₆₇，和GABA-T在所有组织中均显著增加（P  <0.01）。AVM组的结果显示，在安全浓度（SC，0.0039 mg / L）和50的致死浓度下，中央和周围组织中GAD ₆₅，GAD ₆₇和GABA-T的mRNA表达均显着下调。 ％（LC ₅₀，0.039毫克/升），除了GAD mRNA的禁忌₆₅，GAD ₆₇和GABA-T在肌肉在2小时，其大幅下调至0.20±0.02 ^ΔΔ  ×10 ^-2，0.57±0.06 ^ΔΔ  ×10 ^-1和0.44±0.02 ^ΔΔ  ×10 ^-1，分别为（P  <0.01）。