Pyrolysis processes are an alternative to minimize the environmental problem associated to agrifood industrial wastes. The main product resulting from these processes is a high‐value liquid product, called bio‐oil. Recently, the use of comprehensive two‐dimensional liquid chromatography (LC × LC) has been demonstrated as a useful tool to improve the characterization of the water‐soluble phases of bio‐oils, considering their complexity and high water content. However, the precise composition of bio‐oils from different agrifood byproducts is still unknown. In the present study, the qualitative and quantitative screening of eight aqueous phases from different biomasses, not yet reported in the literature, using LC × LC is presented. The two‐dimensional approach was based on the use of two reverse phase separations. An amide column in the first dimension together with a C18 column in the second dimension were employed. Thanks to the use of diode array and mass spectrometry detection, 28 compounds were identified and quantified in the aqueous phase samples with good figures of merit. Samples showed a distinct quali‐quantitative composition and a great predominance of compounds belonging to aldehydes, ketones and phenols, most of them with high polarity.

中文翻译：

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基于二维液相色谱的热解生物油水相的综合定性定量筛选。

热解工艺是最大限度减少与农业食品工业废物相关的环境问题的替代方法。这些过程产生的主要产品是一种高价值的液体产品，称为生物油。最近，考虑到生物油的复杂性和高含水量，综合二维液相色谱 (LC × LC) 的使用已被证明是改善生物油水溶性相表征的有用工具。然而，来自不同农业食品副产品的生物油的确切成分仍然未知。在本研究中，使用 LC × LC 对来自不同生物质的八种水相进行了定性和定量筛选，文献中尚未报道。二维方法基于使用两个反相分离。使用第一维的酰胺柱和第二维的 C18 柱。由于使用二极管阵列和质谱检测，水相样品中的 28 种化合物被鉴定和定量，具有良好的品质因数。样品显示出独特的定性定量组成和属于醛类、酮类和酚类的化合物的很大优势，其中大多数具有高极性。