A simple dark field microscopy technique was used for visualization of blood vessels in normal human renal tissues and carcinoma. Phase contrast condenser ring apt for high power objectives was combined with a 10x objective in order to create a dark field illumination of the specimens examined. The endothelial lining of the vessels had been stained by using CD31 monoclonal antibodies combined with conventional peroxidase immunohistochemistry. The final DAB addition used for this technique induced an intense light scatter in the dark field microscope. This scattered light originating from the endothelial lining made the walls of the bright vessels easily detectable from the dark background.

一种简单的暗场显微镜技术被用于可视化正常人肾组织和癌中的血管。适于高功率物镜的相衬聚光镜环与10倍物镜组合在一起，以对被检样品产生暗场照明。通过使用CD31单克隆抗体结合常规的过氧化物酶免疫组织化学对血管的内皮层进行了染色。用于该技术的最终DAB添加物在暗场显微镜中引起了强烈的光散射。这种来自内皮细胞壁的散射光使明亮的血管壁很容易从黑暗的背景中被检测到。