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An miRNA fingerprint using neural-enriched extracellular vesicles from blood plasma: towards a biomarker for amyotrophic lateral sclerosis/motor neuron disease.
Open Biology ( IF 5.8 ) Pub Date : 2020-06-24 , DOI: 10.1098/rsob.200116
Sandra Anne Banack 1 , Rachael Anne Dunlop 1 , Paul Alan Cox 1
Affiliation  

Biomarkers for amyotrophic lateral sclerosis/motor neuron disease (ALS/MND) are currently not clinically available for disease diagnosis or analysis of disease progression. If identified, biomarkers could improve patient outcomes by enabling early intervention and assist in the determination of treatment efficacy. We hypothesized that neural-enriched extracellular vesicles could provide microRNA (miRNA) fingerprints with unequivocal signatures of neurodegeneration. Using blood plasma from ALS/MND patients and controls, we extracted neural-enriched extracellular vesicle fractions and conducted next-generation sequencing and qPCR of miRNA components of the transcriptome. We here report eight miRNA sequences which significantly distinguish ALS/MND patients from controls in a replicated experiment using a second cohort of patients and controls. miRNA sequences from patient blood samples using neural-enriched extracellular vesicles may yield unique insights into mechanisms of neurodegeneration and assist in early diagnosis of ALS/MND.

中文翻译:

使用来自血浆的神经富集细胞外小泡的miRNA指纹:用于肌萎缩性侧索硬化/运动神经元疾病的生物标记。

肌萎缩性侧索硬化/运动神经元疾病(ALS / MND)的生物标志物目前尚无临床可用于疾病诊断或疾病进展分析。如果被发现,生物标志物可以通过支持早期干预并帮助确定治疗效果来改善患者预后。我们假设神经丰富的细胞外囊泡可以提供具有明确的神经变性特征的microRNA(miRNA)指纹。使用来自ALS / MND患者和对照的血浆,我们提取了神经富集的细胞外囊泡组分,并对转录组的miRNA组分进行了下一代测序和qPCR。我们在此报告了使用第二组患者和对照的重复实验中的八个miRNA序列,这些序列显着区分了ALS / MND患者与对照。
更新日期:2020-06-24
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