The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal-amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.

中文翻译：

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肿瘤细胞迁移三维分析的新颖工作流程。


三维 (3D) 细胞成像中二维分析的局限性损害了生物学研究结果的准确性。在这里，我们报告了一种新颖的 3D 方法来采集、分析和解释肿瘤球体图像。我们对间充质-变形虫转变的研究兴趣导致了工作流程的开发，该工作流程将 3D 数据的生成和分析与即时结构化照明显微镜和新的 ImageJ 插件相结合。