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Brief communication: rapid elimination of egg stickiness using sodium hypochlorite in wels catfish Silurus glanis
Fish Physiology and Biochemistry ( IF 2.5 ) Pub Date : 2020-07-14 , DOI: 10.1007/s10695-020-00843-3
Martin Pšenička 1 , Roman Franěk 1
Affiliation  

The wels catfish Silurus glanis is valuable fish for aquaculture. Its production relies mainly on artificial reproduction. One of the crucial steps determining success of the reproduction is elimination of egg stickiness after fertilization. To date, the catfish egg de-adhesion is usually carried out using proteolytic enzymes. Here, we prove a novel method based on oxidation of the egg surface by means of sodium hypochlorite. An effect of different exposure times and concentrations on the egg adhesiveness and damage was tested in the first trial. The selected concentration of sodium hypochlorite 0.3 mg · l−1 with exposure time 40 s was used for comparison with the conventical de-adhesion method using alcalase treatment. The fertilization and hatching rates reached very satisfactory outcome in both treatments (98.3 ± 0.7% vs 97.5 ± 2.2% and 86.6 ± 8.3% vs 91.3 ± 8.5% in alcalase- and sodium hypochlorite-treated embryos, respectively) without any statistical differences. Thus, the de-adhesion method using sodium hypochlorite can be recommended as a suitable method for wels catfish eggs. The method is simple, cheap, very fast, and the treated eggs are disinfected.



中文翻译:

简讯:用次氯酸钠快速消除鲶鱼 Silurus glanis 中的蛋粘性

鲶鱼Silurus glanis是水产养殖的宝贵鱼类。其生产主要依靠人工繁殖。决定繁殖成功与否的关键步骤之一是消除受精后的卵粘性。迄今为止,鲶鱼卵脱粘通常是使用蛋白水解酶进行的。在这里,我们证明了一种基于次氯酸钠氧化鸡蛋表面的新方法。在第一次试验中测试了不同暴露时间和浓度对鸡蛋粘附性和损伤的影响。次氯酸钠的选定浓度 0.3 mg·l -1使用暴露时间为 40 s 的方法与使用 alcalase 处理的传统脱粘方法进行比较。两种处理的受精率和孵化率都达到了非常令人满意的结果(alcalase 和次氯酸钠处理的胚胎分别为 98.3 ± 0.7% 对 97.5 ± 2.2% 和 86.6 ± 8.3% 对 91.3 ± 8.5%),没有任何统计学差异。因此,可以推荐使用次氯酸钠的脱粘方法作为一种适合鲶鱼卵的方法。该方法简单、便宜、速度非常快,而且处理过的鸡蛋是经过消毒的。

更新日期:2020-07-14
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