The pluripotent state of stem cells depends on the complicated network orchestrated by thousands of factors and genes. Long noncoding RNAs (lncRNAs) are a class of RNA longer than 200 nt without a protein-coding function. Single-cell sequencing studies have identified hundreds of lncRNAs with dynamic changes in somatic cell reprogramming. Accumulating evidence suggests that they participate in the initiation of reprogramming, maintenance of pluripotency, and developmental processes by cis and/or trans mechanisms. In particular, they may interact with proteins, RNAs, and chromatin modifier complexes to form an intricate pluripotency-associated network. In this review, we focus on recent progress in approaches to profiling functional lncRNAs in somatic cell reprogramming and cell differentiation.

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分析与干细胞多能性相关的功能性长链非编码 RNA 的新方法

干细胞的多能状态取决于由数千个因素和基因精心编排的复杂网络。长链非编码 RNA (lncRNA) 是一类长度超过 200 nt 且不具有蛋白质编码功能的 RNA。单细胞测序研究已经确定了数百个在体细胞重编程中具有动态变化的 lncRNA。越来越多的证据表明，它们通过顺式和/或反式机制参与重编程的启动、多能性的维持和发育过程。特别是，它们可能与蛋白质、RNA 和染色质修饰复合物相互作用，形成复杂的多能性相关网络。在这篇综述中，我们重点关注在体细胞重编程和细胞分化中分析功能性 lncRNA 的方法的最新进展。