ABSTRACT: Oxytetracycline (OTC) has been commonly used as an effective antibiotic against various fish bacterial diseases, including vibriosis. In this study, the absorption-enhancing effect of citric acid on oral OTC pharmacokinetics and treatment of artificial Vibrio anguillarum infection was evaluated in juvenile yellowtail Seriola quinqueradiata followed by serum OTC concentration analysis. When 25 mg kg^-1 body weight (BW) OTC was administered in combination with 1250 mg kg^-1 BW citric acid, the serum OTC concentration reached almost the same concentration as that of the group treated with 50 mg kg^-1 BW OTC. This coadministration successfully suppressed mortality due to vibriosis similar to the group treated with 50 mg kg^-1 BW OTC. Conversely, poor efficacy was observed when only 25 mg kg^-1 BW OTC was administered. These results suggest that coadministration of citric acid can be beneficial in reducing the dose of OTC needed for effective treatment, and thus contributes to the goal of reduced use of this antibiotic in aquaculture.

中文翻译：

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柠檬酸的共同给药可以减少感染鳗gu弧菌的黄尾西里拉金丝猴的土霉素剂量。

摘要：土霉素（OTC）通常被用作对抗各种鱼类细菌性疾病（包括弧菌病）的有效抗生素。在这项研究中，评估了柠檬酸对口服OTC药代动力学的吸收增强作用以及对青少年yellow鱼西里罗非鱼（Seriola quinqueradiata）人工弧菌感染的治疗，然后进行了血清OTC浓度分析。当25 mg kg ^-1体重（BW）的OTC与1250 mg kg ^-1 BW柠檬酸联合给药时，血清OTC浓度几乎达到与50 mg kg ^-1体重治疗组相同的浓度。BW OTC。与50 mg kg ^-1 BW OTC治疗组相似，这种共同给药成功地抑制了由玻璃纤维化引起的死亡率。相反，当仅施用25 mg kg ^-1 BW OTC时，观察到疗效差。这些结果表明，柠檬酸的共同给药可以减少有效治疗所需的OTC剂量，从而有助于减少在水产养殖中使用这种抗生素的目标。