ABSTRACT: Discovered in 2013, the chytrid fungus Batrachochytrium salamandrivorans (Bsal) is an emerging amphibian pathogen that causes ulcerative skin lesions and multifocal erosion. A closely related pathogen, B. dendrobatidis (Bd), has devastated amphibian populations worldwide, suggesting that Bsal poses a significant threat to global salamander biodiversity. To expedite research into this emerging threat, we seek to standardize protocols across the field so that results of laboratory studies are reproducible and comparable. We have collated data and experience from multiple labs to standardize culturing practices of Bsal. Here we outline common culture practices including a medium for standardized Bsal growth, standard culturing protocols, and a method for isolating Bsal from infected tissue.

中文翻译：

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分离和维持嗜盐芽孢杆菌培养物。

摘要：乳糜菌Batrachochytrium salamandrivorans（Bsal）于2013年发现，是一种新兴的两栖类病原体，可引起溃疡性皮肤损伤和多灶性糜烂。密切相关的病原体B. dendrobatidis（Bd）破坏了全球两栖动物种群，这表明Bsal对全球sal的生物多样性构成了重大威胁。为了加快对这一新兴威胁的研究，我们寻求使整个领域的协议标准化，以使实验室研究的结果具有可重复性和可比性。我们已收集来自多个实验室的数据和经验，以标准化Bsal的培养实践。在这里，我们概述了常见的培养方法，包括标准化Bsal生长的培养基，标准培养方案以及从感染组织中分离Bsal的方法。