Considering the excessive lipid accumulation status caused by the increased dietary lipid intake in farmed fish, this study aimed to investigate the systemic effect of dietary lipid levels and α-lipoic acid supplementation on nutritional metabolism in zebrafish. A total of 540 male zebrafish (0.17 g) were fed with normal (CT) and high lipid level (HL) diets for 6 weeks, then fed on 1000 mg/kg α-lipoic acid supplementation diets for the second 6 weeks. HL diets did not affect whole fish protein content, but increased ASNS expression (P < 0.05). Dietary α-lipoic acid increased whole fish protein content, and decreased the expressions of protein catabolism-related genes in muscle of high lipid level groups (P < 0.05). Furthermore, HL diets increased the whole fish lipid content and the expressions of gluconeogenesis and lipogenesis-related genes (P < 0.05), and α-lipoic acid counteracted these effects and decreased the whole fish triglyceride and cholesterol contents and expressions of lipogenesis-related genes, with the enhanced expressions of lipolytic genes, especially in high lipid groups (P < 0.05). HL diets did not affect hepatocyte mitochondrial quantity or the mRNA expressions of mitochondrial biogenesis and electron transport chain-related genes; they were significantly increased by dietary α-lipoic acid (P < 0.05). These results indicated that high dietary lipid promotes lipid accumulation, while α-lipoic acid increases protein content in association of enhanced lipid catabolism. Thus, dietary α-lipoic acid supplementation could reduce lipid accumulation under high lipid, which provides a promising new approach in solving the problem of lipid accumulation in farmed fish.

考虑到养殖鱼类日粮脂质摄入量增加引起的过多脂质积累状态，本研究旨在研究日粮脂质水平和α-硫辛酸补充对斑马鱼营养代谢的系统性影响。共有540只斑马鱼（0.17 g）分别饲喂正常（CT）和高脂质水平（HL）饮食6周，然后在第二个6周接受1000 mg / kgα-硫辛酸补充饮食。HL饮食不影响全鱼蛋白质含量，但增加了ASNS的表达（P <  0.05）。饮食中的α-硫辛酸增加了高脂水平人群肌肉中全鱼的蛋白质含量，并降低了蛋白质分解代谢相关基因的表达（P < 0.05）。此外，高脂饮食增加了整条鱼的脂质含量以及糖异生和脂肪形成相关基因的表达（P <  0.05），α-硫辛酸抵消了这些影响，并降低了整条鱼的甘油三酸酯和胆固醇含量以及与脂肪生成相关的基因的表达。 ，与脂解基因的表达增强有关，尤其是在高脂族中（P <  0.05）。高脂饮食不影响肝细胞线粒体数量或线粒体生物发生和电子传输链相关基因的mRNA表达。饮食中的α-硫辛酸会显着增加它们（P < 0.05）。这些结果表明高饮食脂质促进脂质积累，而α-硫辛酸增加蛋白质含量并增强脂质分解代谢。因此，日粮中添加α-硫辛酸可以减少高脂条件下的脂质积累，为解决养殖鱼类的脂质积累问题提供了一种有希望的新方法。