Background: Electroanalytical methods are very functional to detect drugs in pharmaceuticals (tablets, syrups, suppositories, creams, and ointments) and biological samples.

Objective: This study is aimed to make selective, sensitive, simple, fast, and low cost electrochemical analysis of expectorant drug guaifenesin in pharmaceuticals and serum samples.

Methods: Differential pulse adsorptive stripping voltammetric method for determination of guaifenesin on a poly(acridine orange) modified glassy carbon electrode has been developed. Glassy carbon electrode was modified with electropolymerization of the acridine orange monomer for the sensitive determination of guaifenesin. Guaifenesin provided highly reproducible and welldefined irreversible oxidation peaks at +1.125 V and +1.128 V (vs. Ag/AgCl) in the selected supporting electrolyte and human serum samples, respectively.

Results: Under optimized conditions, linear response of peak current on the concentration of guaifenesin has been obtained in the ranges of 2.00×10-7 to 1.00×10-4 M in Britton Robinson buffer solution at pH 7.0 and 4.00×10-7 to 1.00×10-4 M in serum samples. The precision of the method was detected by intraday and inter-day repeatability studies in the supporting electrolyte and serum samples media.

Conclusion: The analytical applicability of the proposed method exhibited satisfying determination results for guaifenesin from pharmaceutical dosage forms (syrup) and human serum samples without any pre-separation procedures.

背景：电分析方法非常适用于检测药物（片剂、糖浆、栓剂、乳膏和软膏）和生物样品中的药物。

目的：本研究旨在对药物和血清样品中的祛痰药物愈创甘油醚进行选择性、灵敏、简单、快速和低成本的电化学分析。

方法：建立了在聚（吖啶橙）修饰的玻碳电极上测定愈创甘油醚的微分脉冲吸附溶出伏安法。用吖啶橙单体电聚合修饰玻碳电极，用于愈创甘油醚的灵敏测定。愈创甘油醚在选定的支持电解质和人血清样品中分别在 +1.125 V 和 +1.128 V（相对于 Ag/AgCl）处提供了高度可重现和明确定义的不可逆氧化峰。

结果：在优化条件下，在 pH 7.0 和 4.00×10-7 的 Britton Robinson 缓冲溶液中，峰值电流对愈创甘油醚浓度的线性响应范围为 2.00×10-7 至 1.00×10-4 M 至血清样品中 1.00×10-4 M。该方法的精密度通过支持电解质和血清样品介质的日内和日间重复性研究来检测。

结论：所提出的方法的分析适用性表明，无需任何预分离程序即可对药物剂型（糖浆）和人血清样品中愈创甘油醚的测定结果令人满意。