In this paper, we have reported a rapid, simple, sensitive, straightforward, and validated method for the concentration determination of curcumin (CUR) in nanoliposomes and plasma using the spectrofluorimetry. For both nanoliposomal formulation and plasma, methanol was used as a solvent to extract the CUR. The excitation and emission wavelengths were set at 423 nm and 527 nm, respectively. The method validation was performed based on International Council for Harmonization (ICH) guidelines, Q2, in which parameters; such as, linearity, precision, accuracy and etc., were determined. The results showed that the calibration curve was linear for CUR concentrations of 0.05 to 0.5 μg /mL with a correlation coefficient of 0.9996. The limit of detection (LOD) and limit of quantification (LOQ) were 0.03 and 0.10 μg/mL, respectively. Liposomal CUR (15 mg/kg) was injected intravenously to mice, and at certain time intervals (1, 3, 6, and 24 h), blood samples were collected. The samples were extracted by methanol and CUR concentrations were detected using a fluorescence spectrophotometer. Results indicated the rate of liposomal formulation decline was slower than free CUR. The results of this study indicated that the validation method based on fluorimetry which was developed here is reliable for the detection of CUR in liposomal formulations and plasma.

中文翻译：

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用于测定纳米脂质体和血浆中姜黄素的荧光分光光度法开发和验证。

在本文中，我们报道了一种使用荧光分光光度法测定纳米脂质体和血浆中姜黄素 (CUR) 浓度的快速、简单、灵敏、直接且经过验证的方法。对于纳米脂质体制剂和血浆，使用甲醇作为溶剂来提取 CUR。激发和发射波长分别设置为 423 nm 和 527 nm。方法验证是根据国际协调委员会 (ICH) 指南 Q2 进行的，其中参数；线性度、精密度、准确度等，进行了测定。结果表明，CUR 浓度为 0.05 至 0.5 μg /mL 时，校准曲线呈线性，相关系数为 0.9996。检测限 (LOD) 和定量限 (LOQ) 分别为 0.03 和 0.10 μg/mL。给小鼠静脉注射脂质体CUR（15 mg/kg），并在一定的时间间隔（1、3、6和24小时）采集血样。用甲醇提取样品，并使用荧光分光光度计检测 CUR 浓度。结果表明脂质体制剂的下降速度比游离 CUR 慢。该研究的结果表明，此处开发的基于荧光测定法的验证方法对于检测脂质体制剂和血浆中的 CUR 是可靠的。