The isolation and culture of murine Bone Marrow-derived Mesenchymal stromal Stem Cells (mBMSCs) have attracted great interest in terms of the pre-clinical applications of stem cells in tissue engineering and regenerative medicine. In addition, culturing mBMSCs is important for studying the molecular mechanisms of bone remodeling using relevant transgenic mice. Several factors have created challenges in the isolation and high-yield expansion of homogenous mBMSCs; these factors include low frequencies of Bone Marrow-derived mesenchymal stromal Stem Cells (BMSCs) in bone marrow, variation among inbred mouse strains, contamination with Haematopoietic Progenitor Cells (HPCs), the replicative senescence phenotype and cellular heterogeneity. In this review, we provide an overview of nearly all protocols used for isolating and culturing mBMSCs with the aim of clarifying the most important guidelines for culturing highly purified mBMSC populations retaining in vitro and in vivo differentiation potential.

小鼠骨髓间充质干细胞 (mBMSCs) 的分离和培养在干细胞在组织工程和再生医学中的临床前应用方面引起了极大的兴趣。此外，培养 mBMSCs 对于使用相关转基因小鼠研究骨重塑的分子机制很重要。有几个因素给同质 mBMSCs 的分离和高产量扩增带来了挑战；这些因素包括骨髓中骨髓来源的间充质干细胞 (BMSC) 的频率低、近交小鼠品系之间的变异、造血祖细胞 (HPC) 的污染、复制衰老表型和细胞异质性。在这次审查中，