12-O-tetradecanoylphorbol-13-acetate (TPA), is a major active constituent of the seed oil of Croton tiglium L., has pharmacological activity for the treatment of acute myeloid leukemia patients. Diethyldithiocarbamate (DTC) is a potent inhibitor of NF-κB show activity of anticancer. In this study, we determined the effect of DTC and TPA in combination on HL-60 cells cultured in vitro and in vivo. In this study, we have shown that DTC and TPA synergistically inhibited the growth of HL-60 cells and strongly induced apoptosis in the cells. Mechanistic studies showed that the combined effects of DTC and TPA were associated with a decrease in Bcl-2. The animal experiment showed that the combination of DTC and TPA more potently inhibited the growth of HL-60 tumors than either agent alone. Our results indicate that the administration of TPA and DTC in combination may be an effective strategy for inhibiting the growth of acute myeloid leukemia cells.

12-O-十四烷酰佛波醇-13-乙酸盐（TPA）是巴豆角豆种子油的主要活性成分，具有治疗急性髓细胞性白血病患者的药理活性。二乙基二硫代氨基甲酸酯（DTC）是一种有效的NF-κB抑制剂，具有抗癌活性。在这项研究中，我们确定了DTC和TPA联合对体外和体内培养的HL-60细胞的作用。在这项研究中，我们已经显示DTC和TPA协同抑制HL-60细胞的生长并强烈诱导细胞凋亡。机理研究表明，DTC和TPA的联合作用与Bcl-2的减少有关。动物实验表明，与单独使用任何一种药物相比，DTC和TPA的结合都更有效地抑制HL-60肿瘤的生长。我们的结果表明，联合使用TPA和DTC可能是抑制急性髓性白血病细胞生长的有效策略。