Genetic mapping and nucleotide diversity of two powdery mildew-resistance loci in melon (Cucumis melo L.).,Phytopathology

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Genetic mapping and nucleotide diversity of two powdery mildew-resistance loci in melon (Cucumis melo L.).
Phytopathology ( IF 2.6 ) Pub Date : 2020-10-21 , DOI: 10.1094/phyto-03-20-0078-r
Cui Haonan _{1,

2} , Ding Zhuo _{1,

2} , Fan Chao _{1,

2,

3} , Zhu Zicheng _{1,

2} , Zhang Hao _{1,

2} , Gao Peng _{1,

2} , Luan Feishi _{1,

2}

Affiliation

Powdery mildew (PM) significantly and negatively affects the yield and quality of melon (Cucumis melo) worldwide. Race 2F is the predominant physiological race of the pathogen Podosphaera xanthii in many regions. We used accessions PMR 6 (P₁; resistant to PM) and M1-7 (P₂; susceptible to PM) to analyze the inheritance of resistance to PM (race 2F). The ratio between resistant and susceptible individuals fits a Mendelian segregation ratio of 13:3 in a total of 256 F₂ individuals and 1:1 in BC₁P₂. The resistance to PM in PMR 6 was governed by two genes: a dominant (AA) gene with an epistatic effect and a recessive gene (bb). Only individuals with aaBB or aaBb genotypes were susceptible to PM. Two PM resistance loci, Pm2.1 and pm12.1, were mapped on chromosomes 2 and 12 by bulked segregant analysis and secondary mapping by quantitative trait loci analysis with 18 markers. A new marker-assisted selection system to identify melon genotypes resistant or susceptible to PM was developed and tested in 93 melon accessions. Nucleotide diversity (π) and fixation index (Fst) for the two PM resistance loci were estimated using resequencing data of 336 melons from three groups: C. melo subsp. agrestis, Cucumis melo subsp. melo, and the intermediate type. The lowest π was observed in C. melo ssp. agrestis, and the highest Fst value was between C. melo ssp. agrestis and C. melo ssp. melo. The findings provide a promising tool that can be used to accelerate breeding for durable resistance to PM.

中文翻译：

瓜（Cucumis melo L.）中两个抗白粉病基因座的遗传图谱和核苷酸多样性。

白粉病（PM）对全世界甜瓜（Cucumis melo L.）的产量和品质均产生显着负面影响。第2F种族是许多地区致病性黄腐病菌的主要生理种族。我们使用了登录号PMR 6（P1；抗PM）和M1-7（P2；易受PM）来分析对PM（种族2F）的抗性遗传。抗性个体和易感个体之间的比例在总共256个F2个人中与孟德尔偏爱比例为13：3，而在BC1P2中则为1：1。PMR 6中对PM的抗性由两个基因控制：具有上位效应的显性（AA）基因和隐性基因（bb）。只有具有aaBB或aaBb基因型的个体容易患PM。两个抗白粉病基因座，Pm2.1和pm12.1，通过批量分离子分析将其定位在2号和12号染色体上，并通过QTL分析与18个标记进行二次定位。开发了一种新的标记辅助选择系统来鉴定对PM抗药性或易感性的甜瓜基因型，并在93个甜瓜品种中进行了测试。使用三个组的336个瓜的重测序数据，估算了两个抗白粉病基因座的核苷酸多样性（π）和固定指数（Fst）。agrestis，黄瓜甜瓜亚种。旋律和中间类型。在C. melo ssp中观察到最低的π。agrestis，并且最高的Fst值介于C. melo ssp之间。agrestis和C.melo ssp。旋律这些发现提供了一种有前途的工具，可用于加速育种以获得对PM的持久抗性。开发了一种新的标记辅助选择系统来鉴定对PM抗药性或易感性的甜瓜基因型，并在93个甜瓜品种中进行了测试。使用三个群体的336个瓜的重测序数据，估算了两个抗白粉病基因座的核苷酸多样性（π）和固定指数（Fst）。agrestis，黄瓜甜瓜亚种。旋律和中间类型。在C. melo ssp中观察到最低的π。agrestis，并且最高的Fst值介于C. melo ssp之间。agrestis和C.melo ssp。旋律这些发现提供了一种有前途的工具，可用于加速育种以获得对PM的持久抗性。开发了一种新的标记辅助选择系统来鉴定对PM抗药性或易感性的甜瓜基因型，并在93个甜瓜品种中进行了测试。使用三个组的336个瓜的重测序数据，估算了两个抗白粉病基因座的核苷酸多样性（π）和固定指数（Fst）。agrestis，黄瓜甜瓜亚种。旋律和中间类型。在C. melo ssp中观察到最低的π。agrestis，并且最高的Fst值介于C. melo ssp之间。agrestis和C.melo ssp。旋律这些发现提供了一种有前途的工具，可用于加速育种以获得对PM的持久抗性。使用三个组的336个瓜的重测序数据，估算了两个抗白粉病基因座的核苷酸多样性（π）和固定指数（Fst）。agrestis，黄瓜甜瓜亚种。旋律和中间类型。在C. melo ssp中观察到最低的π。agrestis，并且最高的Fst值介于C. melo ssp之间。agrestis和C.melo ssp。旋律这些发现提供了一种有前途的工具，可用于加速育种以获得对PM的持久抗性。使用三个组的336个瓜的重测序数据，估算了两个抗白粉病基因座的核苷酸多样性（π）和固定指数（Fst）。agrestis，黄瓜甜瓜亚种。旋律和中间类型。在C. melo ssp中观察到最低的π。agrestis，并且最高的Fst值介于C. melo ssp之间。agrestis和C.melo ssp。旋律这些发现提供了一种有前途的工具，可用于加速育种以获得对PM的持久抗性。

更新日期：2020-12-05

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