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Differentiation of Human Induced Pluripotent Stem Cells into Male Germ Cells
Current Stem Cell Research & Therapy ( IF 2.1 ) Pub Date : 2021-06-30 , DOI: 10.2174/1574888x15666200705214223
Na Zhao 1 , Min Sheng 2 , Xia Wang 1 , Yonghui Li 3 , Maryam Farzaneh 4
Affiliation  

Infertility is defined as not being able to become pregnant or to conceive a child after one year or longer of regular unprotected intercourse. Male infertility refers to a male’s inability to cause pregnancy that can result from deficiencies in semen quality, sperm concentration, or abnormal sperm function. Till now, there are few effective methods for the treatment of a couple with male infertility. In the past few years, stem cell-based therapy as a promising strategy has emerged for the treatment of male infertility. Human Pluripotent Stem Cells (hPSCs) can self-renew and differentiate into any type of cell. Human Embryonic Stem Cells (hESCs) and induced Pluripotent Stem Cells (hiPSCs) are two pluripotent populations that can proliferate and give rise to ectodermal, mesodermal, endodermal, and germ cell lineages. Both undifferentiated hiPSCs and hESCs are powerful candidates for the treatment of male infertility. Generation of male germ cells from hPSCs can provide new mechanistic insights into the regulation of spermatogenesis and have a great opportunity for families with infertility. Therefore, a robust, reproducible, and low-cost culture method that supports hPSCs differentiation into male germ cells is necessary. However, very few studies have focused on the derivation of sperm-like cells from hiPSCs and the details of hPSCs differentiation into male germ cells have not been fully investigated. Therefore, in this review, we focus on the in vitro differentiation potential of hiPSCs into male germ cells.



中文翻译:

人类诱导多能干细胞分化为雄性生殖细胞

不孕症的定义是在一年或更长时间的无保护性交后无法怀孕或怀孕。男性不育是指男性因精液质量、精子浓度或精子功能异常而无法怀孕。目前,治疗男性不育夫妇的有效方法很少。在过去几年中,基于干细胞的疗法作为治疗男性不育症的一种很有前景的策略出现。人类多能干细胞 (hPSC) 可以自我更新并分化为任何类型的细胞。人类胚胎干细胞 (hESC) 和诱导多能干细胞 (hiPSC) 是两个多能细胞群,它们可以增殖并产生外胚层、中胚层、内胚层和生殖细胞谱系。未分化的 hiPSC 和 hESC 都是治疗男性不育症的有力候选者。从 hPSC 产生雄性生殖细胞可以为精子发生的调节提供新的机制见解,并为不育家庭提供了很好的机会。因此,需要一种支持 hPSC 分化为雄性生殖细胞的稳健、可重复和低成本的培养方法。然而,很少有研究关注从 hiPSCs 衍生出精子样细胞,并且没有充分研究 hPSCs 分化为雄性生殖细胞的细节。因此,在这篇综述中,我们关注 hiPSCs 向雄性生殖细胞的体外分化潜能。从 hPSC 产生雄性生殖细胞可以为精子发生的调节提供新的机制见解,并为不育家庭提供了很好的机会。因此,需要一种支持 hPSC 分化为雄性生殖细胞的稳健、可重复和低成本的培养方法。然而,很少有研究关注从 hiPSCs 衍生出精子样细胞,并且没有充分研究 hPSCs 分化为雄性生殖细胞的细节。因此,在这篇综述中,我们关注 hiPSCs 向雄性生殖细胞的体外分化潜能。从 hPSC 产生雄性生殖细胞可以为精子发生的调节提供新的机制见解,并为不育家庭提供了很好的机会。因此,需要一种支持 hPSC 分化为雄性生殖细胞的稳健、可重复和低成本的培养方法。然而,很少有研究关注从 hiPSCs 衍生出精子样细胞,并且没有充分研究 hPSCs 分化为雄性生殖细胞的细节。因此,在这篇综述中,我们关注 hiPSCs 向雄性生殖细胞的体外分化潜能。很少有研究关注从 hiPSC 中衍生出精子样细胞,并且尚未充分研究 hPSC 分化为雄性生殖细胞的细节。因此,在这篇综述中,我们关注 hiPSCs 向雄性生殖细胞的体外分化潜能。很少有研究关注从 hiPSC 中衍生出精子样细胞,并且尚未充分研究 hPSC 分化为雄性生殖细胞的细节。因此,在这篇综述中,我们关注 hiPSCs 向雄性生殖细胞的体外分化潜能。

更新日期:2021-07-26
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