Abstract Bletilla striata polysaccharide (BSP) is the main component of Bletilla striata, which has important pharmacological and pharmacological effects; however, due to the lack of genetic data, the metabolic pathways of BSP remain unclear. For this study, 11 representative resources of B. striata were analyzed, and the BSP contents of the different samples were significantly different; however, the monosaccharide composition of BSP was glucose and mannose. The representative samples were selected to observe their life history in situ, which were then divided and cultured in a greenhouse. Finally, samples from various organs of different plants were combined for transcriptome sequencing using the Illumina system. Our results summarized the BSP metabolic pathway, and we found that there were eight enzyme genes involved in biosynthesis, but these genes showed tissue specificity. Following qRT-PCR validation and comparative analysis, manA showed the highest expression; however, there were significant differences between the two germplasm resources in which the BSP content was significantly different, while UGP2, GPI, PMM, and GMPP had significant differences between the two samples. In summary, this study lays the foundation for further research into BSP metabolism and other physiological processes at the molecular level.

中文翻译：

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白芨（兰科）转录组的从头测序和多糖生物合成相关基因的鉴定

摘要 白芨多糖(BSP)是白芨的主要成分，具有重要的药理和药理作用；然而，由于缺乏遗传数据，BSP的代谢途径尚不清楚。本次研究分析了11种具有代表性的B. striata资源，不同样品的BSP含量存在显着差异；然而，BSP 的单糖成分是葡萄糖和甘露糖。选取有代表性的样本进行原位观察生活史，然后分批在温室中培养。最后，使用 Illumina 系统将来自不同植物的各种器官的样本结合起来进行转录组测序。我们的结果总结了 BSP 代谢途径，我们发现有 8 个酶基因参与生物合成，但这些基因显示出组织特异性。经过 qRT-PCR 验证和比较分析，manA 的表达量最高；然而，两种种质资源之间存在显着差异，其中 BSP 含量存在显着差异，而 UGP2、GPI、PMM 和 GMPP 在两个样品之间存在显着差异。总之，本研究为在分子水平上进一步研究 BSP 代谢和其他生理过程奠定了基础。