Abstract Fish eggs are often excluded from identification analysis since at this stage of development there are few morphological characters. The correct identification of eggs can provide important information about spawning areas of species. The current work aimed to identify fish eggs in the Tibagi and Cinzas Rivers using the DNA barcode to obtain information on richness and diversity, adding to the existing data in the area. Of the 928 sequences analyzed using the BOLD Systems database, 99.78% were able to be identified at a specific level, demonstrating a high success rate for egg identification. The samples resulted in 25 species, 11 families, and 2 orders. Of the 25 species found, more than half (60%) present reproductive migration behavior, indicating that the tributaries of the Capivara reservoir are being used as a migratory route by these species. Eggs of rare and endangered species were found, indicating these tributaries as spawning grounds for these species. The results demonstrate the importance of identifying fish eggs in reservoir-influenced environments to recognize breeding areas of native and endangered species, as well as the importance of the Tibagi and Cinzas Rivers for the maintenance of native fish species in the Paranapanema River.

中文翻译：

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DNA条形码在Paranapanema河流域支流鱼卵鉴定中的应用

摘要 鱼卵通常被排除在鉴定分析之外，因为在这个发育阶段几乎没有形态特征。正确识别卵可以提供有关物种产卵区的重要信息。目前的工作旨在使用 DNA 条形码识别 Tibagi 河和 Cinzas 河中的鱼卵，以获得有关丰富度和多样性的信息，增加该地区现有数据。在使用 BOLD Systems 数据库分析的 928 个序列中，99.78% 能够在特定水平上进行识别，表明鸡蛋识别的成功率很高。样本共25种，11科，2目。在发现的 25 个物种中，超过一半 (60%) 表现出生殖迁移行为，表明 Capivara 水库的支流正被这些物种用作迁徙路线。发现了稀有和濒危物种的卵，表明这些支流是这些物种的产卵地。结果证明了在受水库影响的环境中识别鱼卵以识别本地和濒危物种繁殖区的重要性，以及蒂巴吉河和辛扎斯河对维持巴拉那帕内马河本地鱼类物种的重要性。