Three polysaccharides (SH-1, SH-2 and SH-3) were purified from a brown macroalgea, Sargassum hemiphyllum. The autohydrolysis products from each polysaccharide were separated to three fractions (S fractions as oligomers, L fractions as low molecular weight polysaccharides and H fractions as high molecular weight polysaccharides). Mass spectroscopy of S fractions (SH-1-S, SH-2-S and SH-3-S) showed that these three polymers all contained short stretches of sulfated fucose. The structures of L fractions (SH-1-L, SH-2-L and SH-3-L) were determined by nuclear magnetic resonance (NMR). SH-1-L was composed of two units, unit A (sulfated galactofucan) and unit B (sulfated xylo-glucuronomannan). Unit A contained a backbone of (1, 6-linked β-D-Gal) n₁, (1, 3-linked 4-sulfated α-L-Fuc) n₂, (1, 3-linked 2, 4-di-sulfated α-L-Fuc) n₃, (1, 4-linked α-L-Fuc) n₄ and (1, 3-linked β-D-Gal) n₅, accompanied by some branches, such as sulfated fuco-oligomers, sulfated galacto-oligomers or sulfated galacto-fuco-oligomers. And unit B consisted of alternating 1, 4-linked β-D-glucuronic acid (GlcA) and 1, 2-linked α-D-mannose (Man) with the Man residues randomly sulfated at C6 or branched with xylose (Xyl) at C3. Both SH-2-L and SH-3-L were composed of unit A and their difference was attributed to the ratio of n₁: n₂: n₃: n₄: n₅. Based on monosaccharide analysis, we hypothesize that both SH-1-H and SH-2-H contained unit A and unit B while SH-3-H had a structure similar to SH-3-L. An assessment of anti-complement activities showed that the sulfated galactofucan had higher activities than sulfated galacto-fuco-xylo-glucuronomannan. These results suggest that the sulfated galactofucans might be a good candidate for anti-complement drugs.

三种多糖（SH-1、SH-2 和 SH-3）从棕色巨藻 Sargassum hemiphyllum中纯化出来。来自每种多糖的自水解产物被分成三个部分（S 部分作为低聚物，L 部分作为低分子量多糖和 H 部分作为高分子量多糖）。S 组分（SH-1-S、SH-2-S 和 SH-3-S）的质谱表明这三种聚合物都含有短链硫酸化岩藻糖。L 级分 (SH-1-L、SH-2-L 和 SH-3-L) 的结构通过核磁共振 (NMR) 确定。SH-1-L由两个单元组成，单元A（硫酸化半乳聚糖）和单元B（硫酸化木葡糖甘露聚糖）。单元 A 包含主链 (1, 6-linked β-D-Gal) n ₁ , (1, 3-linked 4-sulfated α-L-Fuc) n₂ , (1, 3-linked 2, 4-di-sulfated α-L-Fuc) n ₃ , (1, 4-linked α-L-Fuc) n ₄ and (1, 3-linked β-D-Gal ) n ₅，伴随着一些分支，例如硫酸化岩藻糖低聚物、硫酸化半乳低聚物或硫酸化半乳岩藻糖低聚物。单元 B 由交替的 1, 4-连接的 β-D-葡萄糖醛酸 (GlcA) 和 1, 2-连接的 α-D-甘露糖 (Man) 组成，其中 Man 残基在 C6 处随机硫酸化或在C3. SH-2-L和SH-3-L均由单元A组成，它们的差异归因于n ₁ : n ₂ : n ₃ : n ₄ : n ₅的比例. 基于单糖分析，我们假设 SH-1-H 和 SH-2-H 均含有单元 A 和单元 B，而 SH-3-H 具有与 SH-3-L 相似的结构。抗补体活性的评估表明，硫酸化半乳聚糖比硫酸化半乳糖-岩藻糖-木糖-葡糖甘露聚糖具有更高的活性。这些结果表明硫酸化半乳聚糖可能是抗补体药物的良好候选者。