Accumulating evidence indicates that oxidative stress and inflammation are implicated in brachial plexus avulsion (BPA). Quercetin has anti-inflammatory, anti-oxidant, anti-apoptotic, and neuroprotective properties. This study investigated the therapeutic efficacy of a temperature-sensitive poly(D,L-lactide-co-glycolide)-poly(ethylene-glycol)-poly(D,L-lactide-co-glycolide) (PLGA-PEG-PLGA) hydrogel sustained-release system of quercetin in BPA. In situ injections of the hydrogel loaded with different concentrations of quercetin were conducted in a rat model of BPA. Significantly reduced reactive oxygen species and interleukin-6 levels in the injured spinal cord 24 h post-surgery, increased number of anterior horn motor and functional neurons in the spinal cord 6 weeks post-surgery, thickened biceps muscle fibres and enlarged endplate area with clear structure, reduced demyelinated peripheral nerves, and significantly increased Terzis grooming test scores were found in the groups with 50 or 100 mg/mL quercetin-loaded hydrogels compared with the control and blank hydrogel groups. In conclusion, the temperature-sensitive quercetin loaded PLGA-PEG-PLGA hydrogel sustained-release system can alleviate oxidative damage and inflammation in the spinal cord, increase neuron survival rate, and promote nerve regeneration and motor function recovery in rats with early BPA. The findings suggest that this drug-loaded hydrogel has potential applications in the clinical treatment of BPA.

中文翻译：

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热敏PLGA-PEG-PLGA水凝胶对槲皮素的局部递送，用于治疗臂丛神经撕脱。

越来越多的证据表明，氧化应激和炎症与臂丛神经撕脱（BPA）有关。槲皮素具有抗炎，抗氧化，抗凋亡和神经保护的特性。这项研究调查了温度敏感的聚（D，L-丙交酯-乙交酯）-聚（乙二醇）-聚（D，L-丙交酯-乙交酯）（PLGA-PEG-PLGA）的治疗功效BPA中槲皮素水凝胶缓释系统。在BPA大鼠模型中原位注射负载了不同浓度槲皮素的水凝胶。术后24 h受伤脊髓的活性氧种类和白介素6水平显着降低，术后6周脊髓前角运动和功能神经元数量增加，与对照组和空白组相比，在装有50或100 mg / mL槲皮素的水凝胶组中，发现二头肌肌肉纤维增厚，端板面积增大，结构清晰，周围神经脱髓鞘减少，Terzis修饰测试得分显着提高。总之，温度敏感的槲皮素负载的PLGA-PEG-PLGA水凝胶缓释系统可以减轻BPA早期大鼠的氧化损伤和脊髓炎症，提高神经元存活率，并促进神经再生和运动功能的恢复。研究结果表明，这种载有药物的水凝胶在BPA的临床治疗中具有潜在的应用。与空白对照组相比，在装有50或100 mg / mL槲皮素的水凝胶组中，发现Terzis修饰测试分数显着提高。总之，温度敏感的槲皮素负载的PLGA-PEG-PLGA水凝胶缓释系统可以减轻BPA早期大鼠的氧化损伤和脊髓炎症，提高神经元存活率，并促进神经再生和运动功能的恢复。研究结果表明，这种载有药物的水凝胶在BPA的临床治疗中具有潜在的应用。与空白对照组相比，在装有50或100 mg / mL槲皮素的水凝胶组中，Terzis修饰测试分数显着提高。总之，温度敏感的槲皮素负载的PLGA-PEG-PLGA水凝胶缓释系统可以减轻BPA早期大鼠的氧化损伤和脊髓炎症，提高神经元存活率，并促进神经再生和运动功能的恢复。研究结果表明，这种载有药物的水凝胶在BPA的临床治疗中具有潜在的应用。增加早期BPA大鼠的神经元存活率，并促进神经再生和运动功能的恢复。研究结果表明，这种载有药物的水凝胶在BPA的临床治疗中具有潜在的应用。增加早期BPA大鼠的神经元存活率，并促进神经再生和运动功能的恢复。研究结果表明，这种载有药物的水凝胶在BPA的临床治疗中具有潜在的应用。