Aim and Scope: Due to the serious toxicological risks and their widespread use, quantitative determination of phthalates in cosmetic products have importance for public health. The aim of this study was to develop a validated simple, rapid and reliable high-performance liquid chromatography (HPLC) method for the determination of phthalates which are; dimethyl phthalate (DMP), diethyl phthalate (DEP), benzyl butyl phthalate (BBP), di-n-butyl phthalate (DBP), di(2- ethylhexyl) phthalate (DEHP), in cosmetic products and to investigate these phthalate (PHT) levels in 48 cosmetic products marketing in Sivas, Turkey.

Materials and Methods: Separation was achieved by a reverse-phase ACE-5 C18 column (4.6 x 250 mm, 5.0 μm). As the mobile phase, 5 mM KH2PO4 and acetonitrile were used gradiently at 1.5 ml min^-1. All PHT esters were detected at 230 nm and the run time was taking 21 minutes.

Results: This method showed the high sensitivity value the limit of quantification (LOQ) values for which are below 0.64 μg mL^-1 of all phthalates. Method linearity was ≥0.999 (r2). Accuracy and precision values of all phthalates were calculated between (-6.5) and 6.6 (RE%) and ≤6.2 (RSD%), respectively. Average recovery was between 94.8% and 99.6%. Forty-eight samples used for both babies and adults were successfully analyzed by the developed method. Results have shown that, DMP (340.7 μg mL^-1 ±323.7), DEP (1852.1 μg mL^-1 ± 2192.0), and DBP (691.3 μg mL^-1 ± 1378.5) were used highly in nail polish, fragrance and cream products, respectively.

Conclusion: Phthalate esters, which are mostly detected in the content of fragrance, cream and nail polish products and our research in general, are DEP (1852.1 μg mL^-1 ± 2192.0), DBP (691.3 μg mL^-1 ± 1378.5) and DMP (340.7 μg mL^-1 ±323.7), respectively. Phthalates were found in the content of all 48 cosmetic products examined, and the most detected phthalates in general average were DEP (581.7 μg mL^-1 + 1405.2) with a rate of 79.2%. The unexpectedly high phthalate content in the examined cosmetic products revealed a great risk of these products on human health. The developed method is a simple, sensitive, reliable and economical alternative for the determination of phthalates in the content of cosmetic products, it can be used to identify phthalate esters in different products after some modifications.

目的和范围：由于存在严重的毒理学风险和广泛使用，化妆品产品中邻苯二甲酸酯的定量测定对公共卫生至关重要。这项研究的目的是开发一种经过验证的简单，快速和可靠的高效液相色谱（HPLC）方法，用于测定邻苯二甲酸酯。化妆品中的邻苯二甲酸二甲酯（DMP），邻苯二甲酸二乙酯（DEP），邻苯二甲酸苄丁酯（BBP），邻苯二甲酸二正丁酯（DBP），邻苯二甲酸二（2-乙基己基）酯（DEHP）并研究这些邻苯二甲酸酯（PHT） ）在土耳其锡瓦斯（Sivas）的48种化妆品营销中的水平。

材料和方法：通过反相ACE-5 C18色谱柱（4.6 x 250 mm，5.0μm）进行分离。作为流动相，以1.5 ml min ^-1的梯度使用5 mM KH2PO4和乙腈。在230 nm处检测到所有PHT酯，运行时间为21分钟。

结果：该方法显示出高灵敏度值，所有邻苯二甲酸酯的定量极限（LOQ）值均低于0.64μgmL ^-1。方法线性≥0.999（r2）。计算出的所有邻苯二甲酸酯的准确度和精密度值分别在（-6.5）和6.6（RE％）和≤6.2（RSD％）之间。平均回收率在94.8％至99.6％之间。通过开发的方法成功地分析了用于婴儿和成人的四十八个样品。结果表明，DMP（340.7μgmL ^-1 ±323.7），DEP（1852.1μgmL ^-1 ±2192.0）和DBP（691.3μgmL ^-1 ±1378.5）在指甲油，香水和乳霜产品中的使用率很高，分别。

结论：邻苯二甲酸酯，通常在香料，奶油和指甲油产品的含量中检测到，我们的研究通常是DEP（1852.1μgmL ^-1 ±2192.0），DBP（691.3μgmL ^-1 ±1378.5）和DMP （340.7μgmL ^-1 ±323.7）。在所检查的所有48种化妆品中都发现邻苯二甲酸盐，而邻苯二甲酸盐含量最高的是普通DEP（581.7μgmL ^-1+ 1405.2）的比率为79.2％。被检查的化妆品中邻苯二甲酸盐含量出乎意料的高表明这些产品对人体健康具有很大的风险。该方法是测定化妆品中邻苯二甲酸酯含量的一种简单，灵敏，可靠，经济的方法，经过一些修改，可用于鉴别不同产品中邻苯二甲酸酯的含量。