Background: Bioremediation has taken its call for removing pollutants for years. The oilcontaminated surroundings are majorly hazardous for sustaining life, but a great contribution to nature in the form of microorganisms. The complex carbon-hydrogen chain has served as classic raw material to chemical industries, which has perked up the hydrocarbon waste. Microbial remediation has been thus, focused to deal with the lacuna, where the new addition to this category is Microbacterium species.

Objectives: The identification and characterization of lipopeptide biosurfactant producing Microbacterium spp. isolated from brackish river water.

Methods: The strain was isolated from an oil-contaminated lake. The strain was tested with all the other isolated species for oil degradation using screening protocols such as haemolysis, oil spread assay, BATH, E24, etc. The produced biosurfactant was extracted by acid precipitation, followed by solvent recovery. The strain with maximum potential was sequenced and was subjected to phylogeny assessment using in silico tools.

Results: Novel Microbacterium species produce the extracellular biosurfactant. The surface tension of Microbacterium was found to be 32mN/m, indicates its powerful surface tension-reducing property. The strain was optimized for the production of biosurfactant and the best results were obtained with sucrose (2%) and yeast extract (3%) medium at 7 pH and 40°C temperature.

Conclusion: The isolate was confirmed to be a novel Microbacterium species that could produce 0.461 gm biosurfactant in 100 ml of the medium throughout a life cycle and novel strain of isolate was deposited to NCBI as Microbacterium spp. ANSKSLAB01 using an accession number: KU179507.

背景：生物修复已经呼吁去除污染物已有多年历史。受油污染的环境对于维持生命特别危险，但以微生物的形式对自然有很大贡献。复杂的碳氢链一直是化工行业的经典原材料，化工行业充斥着碳氢化合物的浪费。因此，微生物修复一直集中于处理空洞，其中新增加的是细菌种类。

目的：鉴定和鉴定产脂肽生物表面活性剂的微细菌属菌种。从咸淡的河水中分离。

方法：从油污染的湖中分离出该菌株。使用诸如溶血，涂油分析，BATH，E24等筛选方案，对所有其他分离的菌株进行了油降解测试。通过酸沉淀提取产生的生物表面活性剂，然后回收溶剂。对具有最大潜力的菌株进行测序，并使用计算机软件进行系统发育评估。

结果：新的微生物菌种产生细胞外生物表面活性剂。发现微细菌的表面张力为32mN / m，表明其强大的表面张力降低性能。对菌株进行了优化以生产生物表面活性剂，并在7 pH和40°C温度下用蔗糖（2％）和酵母提取物（3％）培养基获得了最佳结果。

结论：该分离株被证实是一种新型的细菌，可以在整个生命周期中在100 ml的培养基中产生0.461 gm的生物表面活性剂，并且该新型分离株以Microbacter spp的形式沉积到NCBI中。ANSKSLAB01，登录号：KU179507。