BACKGROUND Biodiversity screens and phylogenetic studies are dependent on reliable DNA sequences in public databases. Biological collections possess vouchered specimens with a traceable history. Therefore, DNA sequencing of samples available at institutional collections can greatly contribute to taxonomy, and studies on evolution and biodiversity. METHODS We sequenced part of the glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) and the SSU rRNA (V7/V8) genes from 102 trypanosomatid cultures, which are available on request at www.colprot.fiocruz.br. OBJECTIVE The main objective of this work was to use phylogenetic inferences, using the obtained DNA sequences and those from representatives of all Trypanosomatidae genera, to generate phylogenetic trees that can simplify new isolates screenings. FINDINGS A DNA sequence is provided for the first time for several isolates, the phylogenetic analysis allowed the classification or reclassification of several specimens, identification of candidates for new genera and species, as well as the taxonomic validation of several deposits. MAIN CONCLUSIONS This survey aimed at presenting a list of validated species and their associated DNA sequences combined with a short historical overview of each isolate, which can support taxonomic and biodiversity research and promote culture collections.

中文翻译：

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通过条形码分析锥虫分类学中的歧义。

背景技术生物多样性筛选和系统发育研究依赖于公共数据库中可靠的DNA序列。生物收藏品拥有具有可追溯历史的有凭证标本。因此，对机构收藏的样本进行 DNA 测序可以极大地促进分类学以及进化和生物多样性的研究。方法 我们对来自 102 个锥虫培养物的糖体甘油醛磷酸脱氢酶 (gGAPDH) 和 SSU rRNA (V7/V8) 基因的部分进行了测序，这些基因可在 www.colprot.fiocruz.br 上索取。目的 这项工作的主要目的是利用获得的 DNA 序列和所有锥虫科属代表的 DNA 序列进行系统发育推断，生成可以简化新分离株筛选的系统发育树。研究结果 首次提供了多个分离株的 DNA 序列，系统发育分析允许对多个样本进行分类或重新分类，识别新属和新种的候选者，以及对多个沉积物进行分类学验证。主要结论 这项调查旨在提供一份经过验证的物种及其相关 DNA 序列的清单，并结合每个分离株的简短历史概述，这可以支持分类学和生物多样性研究并促进培养物收藏。