SIGNIFICANCE Worldwide, ∼460  million people suffer from disabling hearing impairment. Many of these patients are still not sufficiently supplied with currently available auditory technologies. Optical stimulation of the hearing organ offers a promising alternative for a new generation of auditory prostheses. AIM To assess the biocompatibility margins of our laser pulse amplitude strategy in vitro, we designed a protocol and present the effects on normal human dermal fibroblasts, human chondrocytes, and human osteoblasts. APPROACH Laser pulses of 532 nm were applied over 120 s using our laser pulse amplitude modulation strategy. We then assessed cell viability and cytotoxicity through fluorescence staining and quantitative polymerase chain reaction-analysis regarding 84 key player-genes for cytotoxicity and stress response. RESULTS The first in vitro biocompatibility margins for our stimulation parameters applied to cells of the peripheral hearing organ were between 200 and 223 mW (3348  J/cm2). After irradiation with a subphototoxic laser power of 199 mW (2988  J/cm2), only the fibroblasts showed a significant upregulation of GADD45G. CONCLUSION Further studies are underway to optimize parameters for the optoacoustic stimulation of the auditory system. Our protocol and results on laser-tissue interactions can be useful for translational laser applications in various other irradiated biological tissues.

中文翻译：

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用于开发基于激光的助听器的光声刺激策略的细胞毒性研究。

意义在全球范围内，约有 4.6 亿人患有听力障碍。这些患者中的许多人仍然没有充分利用当前可用的听觉技术。听觉器官的光刺激为新一代听觉假体提供了一种有前途的替代方案。目的为了在体外评估我们的激光脉冲幅度策略的生物相容性边际，我们设计了一个协议并展示了对正常人真皮成纤维细胞、人软骨细胞和人成骨细胞的影响。方法 使用我们的激光脉冲幅度调制策略在 120 秒内施加 532 nm 的激光脉冲。然后，我们通过荧光染色和定量聚合酶链反应分析评估了细胞活力和细胞毒性，分析了 84 个细胞毒性和应激反应的关键参与者基因。结果 我们应用于外周听力器官细胞的刺激参数的第一个体外生物相容性边际在 200 到 223 mW (3348 J/cm2) 之间。在用 199 mW (2988 J/cm2) 的亚光毒性激光功率照射后，只有成纤维细胞显示出 GADD45G 的显着上调。结论正在进行进一步的研究以优化听觉系统的光声刺激参数。我们关于激光组织相互作用的协议和结果可用于各种其他辐照生物组织中的平移激光应用。只有成纤维细胞显示出 GADD45G 的显着上调。结论正在进行进一步的研究以优化听觉系统的光声刺激参数。我们关于激光组织相互作用的协议和结果可用于各种其他辐照生物组织中的平移激光应用。只有成纤维细胞显示出 GADD45G 的显着上调。结论正在进行进一步的研究以优化听觉系统的光声刺激参数。我们关于激光组织相互作用的协议和结果可用于各种其他辐照生物组织中的平移激光应用。