Botryosphaeria dieback is one of the most significant grapevine trunk diseases that affects the sustainability of the vineyards and provokes economic losses. The causal agents, Botryosphaeriaceae species, live in and colonize the wood of the perennial organs causing wood necrosis. Diseased vines show foliar symptoms, chlorosis, or apoplexy, associated to a characteristic brown stripe under the bark. According to the susceptibility of the cultivars, specific proteins such as PR-proteins and other defense-related proteins are accumulated in the brown stripe compared with the healthy woody tissues. In this study, we enhanced the characterization of the brown stripe and the healthy wood by obtaining a metabolite profiling for the three cultivars Chardonnay, Gewurztraminer, and Mourvèdre to deeper understand the interaction between the Botryosphaeria dieback pathogens and grapevine. The study confirmed a specific pattern according to the cultivar and revealed significant differences between the brown stripe and the healthy wood, especially for phytochemical and lipid compounds. This is the first time that such chemical discrimination was made and that lipids were so remarkably highlighted in the interaction of Botryosphaeriaceae species and grapevine. Their role in the disease development is discussed.

灰霉病是最重要的葡萄干病之一，它影响葡萄园的可持续性并造成经济损失。病原体（Botryosphaeriaceae物种）生活在多年生器官的木材中并在其中定殖，导致木材坏死。患病的葡萄藤表现出叶状症状，萎黄或中风，与树皮下的特征性棕色条纹有关。根据品种的敏感性，与健康的木质组织相比，特定的蛋白质（例如PR蛋白和其他防御相关蛋白）会在棕色条纹中积累。在这项研究中，我们通过获得三个栽培品种霞多丽，Gewurztraminer的代谢物谱，增强了棕色条纹和健康木材的特性。和穆维德（Mourvèdre）更深入地了解了灰霉病菌病原菌和葡萄之间的相互作用。该研究证实了根据栽培品种的特定模式，并揭示了棕色条纹和健康木材之间的显着差异，尤其是在植物化学和脂质化合物方面。这是第一次进行这种化学鉴别，并且在葡萄孢菌科物种和葡萄的相互作用中如此突出地突出了脂质。讨论了它们在疾病发展中的作用。这是首次进行这种化学鉴别，并且在葡萄球菌属物种和葡萄的相互作用中如此突出地突出了脂质。讨论了它们在疾病发展中的作用。这是首次进行这种化学鉴别，并且在葡萄球菌属物种和葡萄的相互作用中如此突出地突出了脂质。讨论了它们在疾病发展中的作用。