Dual-enzyme and NADPH co-embedded organic–inorganic hybrid nanoflowers prepared using biomimetic mineralization for the asymmetric synthesis of (R)-(−)-pantolactone,Reaction Chemistry & Engineering

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Dual-enzyme and NADPH co-embedded organic–inorganic hybrid nanoflowers prepared using biomimetic mineralization for the asymmetric synthesis of (R)-(−)-pantolactone
Reaction Chemistry & Engineering ( IF 3.4 ) Pub Date : 2020-08-12 , DOI: 10.1039/d0re00158a
Pengfei Cheng _{1,

2,

3,

4,

5} , Manman Tang _{4,

5,

6,

7} , Zhiji Chen _{4,

5,

6,

7} , Wen Liu _{4,

5,

6,

7} , Xinpeng Jiang _{1,

2,

3,

4,

5} , Xiaolin Pei _{1,

2,

3,

4,

5} , Weike Su _{1,

2,

3,

4,

5}

Affiliation

An effective biocatalytic cascade was developed by encapsulating a conjugated polyketone reductase (CPR), glucose dehydrogenase (GDH) and the coenzyme NADP⁺ in organic–inorganic hybrid nanoflowers (hNFs) for the asymmetric reduction of ketopantolactone (KPL) to synthesize (R)-(−)-pantolactone [(R)-PL]. When CduCPR and TgGDH were confined in the SA-coated hNFs [CduCPR/TgGDH@Ca₃(PO₄)₂], both of them had excellent reusability and high stability. Notably, the SA-coated hNF reactor successfully catalyzed the asymmetric synthesis of (R)-PL, which exhibited satisfactory stereoselectivity and promoted reusability in repeated batches. The SA coating functioned to competently immobilize the coenzyme NADP⁺ on the CduCPR/TgGDH@Ca₃(PO₄)₂ hNFs, which maintained the maximum bioactivity to synthesize (R)-PL without exogenous coenzyme addition. Consequently, the facile immobilization method should be useful as a common approach for multi-enzyme cascade reactions and has potential applications in the biocatalytic industry.

中文翻译：

使用仿生矿化制备的双酶和NADPH共嵌入的有机-无机杂化纳米花，用于（R）-（-）-泛内酯的不对称合成

通过将共轭聚酮还原酶（CPR），葡萄糖脱氢酶（GDH）和辅酶NADP ⁺封装在有机-无机杂化纳米花（hNFs）中，以有效地不对称还原酮戊内酯（KPL）来合成（R）-，从而开发出有效的生物催化级联反应（-）-泛内酯[（R）-PL]。当CDU CPR和Tg的GDH在SA-涂覆hNFs [只限于CDU CPR / Tg的GDH @的Ca ₃（PO ₄）₂ ]，它们两者都具有极佳的可重用性和高稳定性。值得注意的是，SA包覆的hNF反应器成功催化了（R）-PL，其表现出令人满意的立体选择性并提高了重复批次的可重复使用性。所述SA涂层起作用胜任固定辅酶NADP ⁺在CDU CPR / Tg的GDH @的Ca ₃（PO ₄）_2个hNFs，其保持了最大的生物活性来合成（- [R）-PL没有外源辅酶加成。因此，简便的固定化方法应可用作多酶级联反应的常用方法，并在生物催化工业中具有潜在的应用。

更新日期：2020-09-29

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