Infant cardiac surgery with cardiopulmonary bypass results in decreased circulating alkaline phosphatase that is associated with poor postoperative outcomes. Bovine intestinal alkaline phosphatase infusion represents a novel therapy for post-cardiac surgery organ injury. However, the effects of cardiopulmonary bypass and bovine-intestinal alkaline phosphatase infusion on tissue-level alkaline phosphatase activity/expression are unknown. Infant pigs (n = 20) underwent cardiopulmonary bypass with deep hypothermic circulatory arrest followed by four hours of intensive care. Seven control animals underwent mechanical ventilation only. Cardiopulmonary bypass/deep hypothermic circulatory arrest animals were given escalating doses of bovine intestinal alkaline phosphatase infusion (0-25 U/kg/hr.; n = 5/dose). Kidney, liver, ileum, jejunum, colon, heart and lung were collected for measurement of tissue alkaline phosphatase activity and mRNA. Tissue alkaline phosphatase activity varied significantly across organs with the highest levels found in the kidney and small intestine. Cardiopulmonary bypass with deep hypothermic circulatory arrest resulted in decreased kidney alkaline phosphatase activity and increased lung alkaline phosphatase activity, with no significant changes in the other organs. Alkaline phosphatase mRNA expression was increased in both the lung and the ileum. The highest dose of bovine intestinal alkaline phosphatase resulted in increased kidney and liver tissue alkaline phosphatase activity. Changes in alkaline phosphatase activity after cardiopulmonary bypass with deep hypothermic circulatory arrest and bovine intestinal alkaline phosphatase delivery are tissue specific. Kidneys, lung, and ileal alkaline phosphatase appear most affected by cardiopulmonary bypass with deep hypothermic circulatory arrest and further research is warranted to determine the mechanism and biologic importance of these changes.

中文翻译：

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深低温停循环体外循环实验猪模型中的组织碱性磷酸酶活性和表达。

体外循环的婴儿心脏手术导致循环碱性磷酸酶降低，这与术后预后不良有关。牛肠碱性磷酸酶输注代表了心脏手术后器官损伤的新疗法。然而，体外循环和牛肠碱性磷酸酶输注对组织水平碱性磷酸酶活性/表达的影响尚不清楚。婴儿猪 (n = 20) 接受了体外循环和深度低温停循环，然后进行了 4 小时的重症监护。七只对照动物仅接受机械通气。给体外循环/深低温停循环动物以递增剂量的牛肠碱性磷酸酶输注（0-25 U/kg/hr.；n = 5/dose）。肾、肝、回肠、空肠、结肠、收集心脏和肺用于测量组织碱性磷酸酶活性和mRNA。组织碱性磷酸酶活性在肾脏和小肠中最高水平的器官之间存在显着差异。深低温停循环的体外循环导致肾脏碱性磷酸酶活性降低和肺碱性磷酸酶活性增加，其他器官无明显变化。碱性磷酸酶 mRNA 表达在肺和回肠中均增加。最高剂量的牛肠碱性磷酸酶导致肾脏和肝脏组织碱性磷酸酶活性增加。深低温停循环和牛肠碱性磷酸酶递送体外循环后碱性磷酸酶活性的变化是组织特异性的。