The declining rate of male fertility is a growing concern. Tributyltin (TBT) is a well-known endocrine disruptor (ED), that induces imposex in female gastropods and is widely used in various industrial applications. The aim of this study was to evaluate the toxic effects of TBT on the testes of adult albino rats and the possible role of omega-3. Forty two adult male albino rats were divided into five groups; control group (Group I) and four experimental groups: omega-3 treated group, TBT treated group, TBT & omega-3 treated group and follow up group. At the end of the study, the rats were subjected to biochemical, histological, immunohistochemical staining for Ki-67 and seminal examinations. Our results clarfied that TBT induced a significant decrease in testosterone, FSH, LH and serum glutathione peroxidase levels and a significant increase in the serum Malondialdehyde as compared to the control group. Tributyltin induced disorganization and shrinkage of seminiferous tubules, apoptosis, cellular damage and marked reduction in the germinal epithelium. A significant decrease in the cell proliferation and arrested spermatogenesis were also detected. Seminal analysis of TBT group showed a significant affection of all parameters as compared to other groups. Omega-3 ameliorated all of these hazardous effects. Follow up group still showed toxic effects. In conclusion, TBT has a toxic effect on the testis. Increased testicular oxidative stress, cellular damage and arrest of spermatogenesis with attenuation in antioxidant defenses are all contributing factors. Omega-3 can protect against TBT induced reproductive toxicity.

男性生育率的下降越来越令人担忧。三丁基锡 (TBT) 是一种众所周知的内分泌干扰物 (ED)，可在雌性腹足类动物中诱导强迫症，并广泛用于各种工业应用。本研究的目的是评估 TBT 对成年白化大鼠睾丸的毒性作用以及 omega-3 的可能作用。42只成年雄性白化大鼠被分成5组；对照组（I组）和四个实验组：omega-3治疗组、TBT治疗组、TBT&omega-3治疗组和随访组。在研究结束时，对大鼠进行生化、组织学、免疫组织化学染色以进行 Ki-67 和精液检查。我们的结果表明，TBT 导致睾酮、FSH、与对照组相比，LH 和血清谷胱甘肽过氧化物酶水平以及血清丙二醛显着增加。三丁基锡诱导生精小管的解体和收缩、细胞凋亡、细胞损伤和生发上皮的显着减少。还检测到细胞增殖显着减少和精子发生停滞。与其他组相比，TBT 组的精液分析显示所有参数的显着影响。Omega-3 改善了所有这些有害影响。随访组仍显示毒性作用。总之，TBT对睾丸有毒性作用。睾丸氧化应激增加、细胞损伤和精子发生停滞以及抗氧化防御减弱都是促成因素。Omega-3 可以防止 TBT 引起的生殖毒性。