Multifunctional pro-inflammatory cytokine CXCL8 is a small peptide of 8–10 kDa in size and it functions as a monomer or dimer. CXCL8 harbors 2 disulfide bonds for its stability. Although production of the CXCL8 protein in a large quantity in both mammalian and bacterial systems has been reported, the processes are complicated and lengthy. Here, we develop a new bacterial expression system for recombinant CXCL8 and simplify the purification system to yield a high amount of protein quickly. The purified CXCL8 protein from our new system develops a crystal structure that is identical to that produced through the mammalian expression system. Thus, we have established a simple and efficient recombinant CXCL8-producing system, which can be easily operated and is suitable to those requiring a large quantity of CXCL8.

中文翻译：

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在大肠杆菌中生产重组人 CXCL8 的简单有效系统。

多功能促炎细胞因子 CXCL8 是一种大小为 8-10 kDa 的小肽，它作为单体或二聚体发挥作用。CXCL8 具有 2 个二硫键以保证其稳定性。尽管已经报道了在哺乳动物和细菌系统中大量生产 CXCL8 蛋白，但该过程复杂而漫长。在这里，我们为重组 CXCL8 开发了一种新的细菌表达系统，并简化了纯化系统以快速产生大量蛋白质。来自我们新系统的纯化 CXCL8 蛋白形成了与通过哺乳动物表达系统产生的晶体结构相同的晶体结构。因此，我们建立了一个简单高效的重组CXCL8生产系统，该系统操作方便，适用于需要大量CXCL8的人群。