SummaryThe present study evaluated the effect of the aqueous extract from leaves ofE.speciosaon some physiological and biochemical parameters of reproduction and the onset of puberty in pregnant mare serum gonadotropin (PMSG)-primed immature female rats. High pressure liquid chromatography (HPLC) was used to quantify the phenolic compounds in the methanol/methylene chloride (1:1) extract, the ethanolic and ethyl acetate fractions and the aqueous residue ofE.speciosa.E.speciosa(0, 8, 32 or 64 mg/kg) were administered for 15 days to 24 non-PMSG-primed and 24 primed rats with 0.01 IU of PMSG. At the end of the treatment period, animal were sacrificed and their body, ovarian, uterine weight, ovarian protein or cholesterol level, as well as data on puberty onset were recorded. Of the 16 polyphenolic compounds quantitatively revealed in the extracts and fractions ofE.speciosaafter HPLC analysis, quercetin, rutin, apigenin and eugenol were the most abundant. Non-primed rats showed a significant increase (P< 0.05) in the uterine relative weight at the dose of 8 mg/kg when compared with the other treatments. The uterine proteins and the ovarian cholesterol (P< 0.05), respectively, showed a reduction at doses of 64 mg/kg and 32 mg/kg in non-primed rats. However in PMSG-primed rats, a significant decrease (P< 0.05) was observed in ovarian cholesterol at 64 mg/kg. In conclusion,E.speciosapotentializes the PMSG-inducing effect on folliculogenesis in PMSG-primed rats.

中文翻译：

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Eremomastax 对 PMSG 致敏未成熟大鼠某些生理生化参数的 PMSG 诱导作用具有特异性

总结本研究评估了叶水提取物的效果。乙.物种妊娠母马血清促性腺激素 (PMSG) 引发的未成熟雌性大鼠生殖和青春期开始的一些生理生化参数。使用高压液相色谱 (HPLC) 对甲醇/二氯甲烷 (1:1) 提取物中的酚类化合物、乙醇和乙酸乙酯部分以及水性残留物进行定量。乙.物种.乙.物种（0、8、32 或 64 mg/kg）对 24 只非 PMSG 致敏大鼠和 24 只致敏大鼠施用 0.01 IU PMSG，持续 15 天。在治疗期结束时，处死动物并记录它们的身体、卵巢、子宫重量、卵巢蛋白或胆固醇水平以及青春期开始的数据。在 16 种多酚化合物的提取物和馏分中定量显示乙.物种经HPLC分析，槲皮素、芦丁、芹菜素和丁香酚含量最多。未启动的大鼠表现出显着增加（磷< 0.05) 与其他治疗相比，剂量为 8 mg/kg 时的子宫相对重量。子宫蛋白和卵巢胆固醇（磷< 0.05) 分别显示在 64 mg/kg 和 32 mg/kg 的剂量下在未致敏大鼠中减少。然而，在 PMSG 引发的大鼠中，显着下降（磷< 0.05) 在 64 mg/kg 的卵巢胆固醇中观察到。综上所述，乙.物种潜在的 PMSG 诱导对 PMSG 致敏大鼠卵泡发生的作用。