ABSTRACT

S. aureus and S. epidermidis are the most common bacteria responsible for causing implant infections. We propose that 3D-printed antimicrobial biomedical devices are a rational solution for the prevention of infection. 3D printed polylactic acid (PLA) constructs were alkali-treated to increase hydrophilicity and functionalized using a suspension of Zinc/HNTs-Ag-Chitosan Oligosaccharide Lactate (ZnHNTs-Ag-COS). PLA constructs were analyzed using scanning electron microscopy and Fourier-transform infrared spectroscopy (FTIR). The antimicrobial potential was assessed using agar diffusion and biofilm assays. The surface of 3D printed PLA constructs were chemically modified to increase hydrophilicity and suspensions of COS-ZnHNTs-Ag were adsorbed on the construct surface. Surface adsorption of ZnHNTs-Ag-COS on PLA printed constructs was determined to be a function of relative pore size. Morphological surface characterization confirmed the presence of the suspension coatings on the constructs, and FTIR analysis confirmed the presence of COS-ZnHNTs-Ag coatings. Growth inhibition studies validated the antimicrobial properties of PLA constructs.

 抽象的

金黄色葡萄球菌和表皮葡萄球菌是引起种植体感染的最常见细菌。我们认为3D打印抗菌生物医学设备是预防感染的合理解决方案。 3D 打印的聚乳酸 (PLA) 结构经过碱处理以增加亲水性，并使用锌/HNTs-Ag-壳寡糖乳酸盐 (ZnHNTs-Ag-COS) 悬浮液进行功能化。使用扫描电子显微镜和傅里叶变换红外光谱 (FTIR) 对 PLA 结构进行分析。使用琼脂扩散和生物膜测定评估抗菌潜力。 3D 打印的 PLA 结构的表面经过化学修饰以增加亲水性，并且 COS-ZnHNTs-Ag 的悬浮液被吸附在结构表面上。 ZnHNTs-Ag-COS 在 PLA 印刷结构上的表面吸附被确定为相对孔径的函数。形态表面表征证实了结构上存在悬浮涂层，FTIR 分析证实了 COS-ZnHNTs-Ag 涂层的存在。生长抑制研究验证了 PLA 结构的抗菌特性。