ABSTRACT

We sought to investigate the expression characteristics of adiponectin (APN) and receptor activator of nuclear factor kappa B ligand (RANKL) in the periodontal tissues of rats with periodontitis and the effect on vascular calcification. Thirty-six Sprague Dawley rats were randomly divided into a normal control group, periodontitis group, and vascular calcification group. Western blotting was employed to detect the expressions of APN and RANKL in the periodontal tissues of rats. Calcium content and alkaline phosphatase activity in the vascular tissue of rats were determined. Compared to the normal control group, the periodontitis and vascular calcification groups presented lower expressions of APN and RANKL. The expression of APN in the periodontal tissues of rats in the vascular calcification group was significantly lower than that in the periodontitis group. As for the calcium content and alkaline phosphatase activity in rats’ vascular tissues, the vascular calcification group displayed significantly higher rates than the other two groups, whereas the periodontitis group presented lower calcium content and alkaline phosphatase activity than the normal control group. APN and RANKL may be involved in the process of periodontitis and vascular calcification in rats, and some kinds of relationship may exist between periodontitis and vascular calcification.

摘要

我们试图调查脂联素（APN）和核因子κB配体（RANKL）受体激活剂在牙周炎大鼠牙周组织中的表达特征以及对血管钙化的影响。将36只Sprague Dawley大鼠随机分为正常对照组，牙周炎组和血管钙化组。Western blotting检测APN和RANKL在大鼠牙周组织中的表达。测定大鼠血管组织中的钙含量和碱性磷酸酶活性。与正常对照组相比，牙周炎和血管钙化组的APN和RANKL表达较低。血管钙化组大鼠牙周组织中APN的表达明显低于牙周炎组。至于大鼠血管组织的钙含量和碱性磷酸酶活性，血管钙化组显示出明显高于其他两组的比率，而牙周炎组的钙含量和碱性磷酸酶活性则低于正常对照组。APN和RANKL可能参与了大鼠牙周炎和血管钙化的过程，并且牙周炎与血管钙化之间可能存在某种关系。而牙周炎组的钙含量和碱性磷酸酶活性低于正常对照组。APN和RANKL可能参与了大鼠牙周炎和血管钙化的过程，并且牙周炎与血管钙化之间可能存在某种关系。而牙周炎组的钙含量和碱性磷酸酶活性低于正常对照组。APN和RANKL可能参与了大鼠牙周炎和血管钙化的过程，并且牙周炎与血管钙化之间可能存在某种关系。