Brevibacillus offers great potential as a recombinant protein expression host because of its exceptional abilities to synthesize and excrete proteins and its low extracellular protease activity. Despite these strengths, effective recombinant expression strategies are still the key to achieving high-level expression of recombinant proteins in Brevibacillus due to individual differences among strains and target proteins. Many strategies have been developed to improve recombinant protein expression in Brevibacillus. This review begins by introducing the processes used to establish and apply the Brevibacillus expression system, and then critically discusses the strategies available for improving recombinant protein expression in Brevibacillus, including optimization of the host and the expression vector, co-expression of a fusion partner or foldase, and optimization of the fermentation process. Finally, the prospects for further improvement of recombinant protein expression based on Brevibacillus are also discussed. This review is intended to provide a strategic reference for scientists wanting to improve the expression of a specific recombinant protein in Brevibacillus or other expression systems.

短杆菌具有作为重组蛋白表达宿主的巨大潜力，因为它具有出色的合成和排泄蛋白的能力以及低的细胞外蛋白酶活性。尽管有这些优点，但是由于菌株和靶蛋白之间的个体差异，有效的重组表达策略仍然是在短杆菌中实现重组蛋白高水平表达的关键。已经开发了许多策略来改善短杆菌中重组蛋白的表达。这篇综述首先介绍了用于建立和应用短杆菌表达系统的过程，然后批判性地讨论了可用于改善重组蛋白表达的策略。短杆菌，包括宿主和表达载体的优化，融合伴侣或折叠酶的共表达以及发酵过程的优化。最后，还讨论了进一步改进基于短杆菌的重组蛋白表达的前景。这篇综述旨在为希望改善短杆菌或其他表达系统中特定重组蛋白表达的科学家提供战略参考。