Abstract

Amikacin (AMK) is one of the commonly used aminoglycoside antibiotics, introduced for clinical use in patients suffering from bacterial infections especially life-threatening gram-negative infections. Due to lack of chromophore in the molecule, the detection of AMK during analysis is a challenge. Thus, pre and post-column derivatization techniques are generally used for AMK estimation. This review focuses on different analytical methods used for detection and quantification of AMK in pure or fixed dose combination pharmaceutical formulations and biological samples. Various reported methods described in the literature include high-performance liquid chromatography techniques, pulsed electrochemical detection techniques, Chemiluminescence techniques, Capillary electrophoresis and immunological methods. High-performance-liquid-chromatography based methods with UV/Vis spectrophotometric, fluorescence and mass spectrometric detection are the most prevailing methods employed for the analysis of AMK. This review could be of significant importance in the area of future AMK analytical method development studies.

摘要

阿米卡星 (AMK) 是一种常用的氨基糖苷类抗生素，被引入临床用于患有细菌感染，尤其是危及生命的革兰氏阴性菌感染的患者。由于分子中缺乏发色团，因此在分析过程中检测 AMK 是一项挑战。因此，柱前和柱后衍生技术通常用于 AMK 估计。本综述重点介绍了用于检测和定量纯或固定剂量组合药物制剂和生物样品中 AMK 的不同分析方法。文献中描述的各种报道方法包括高效液相色谱技术、脉冲电化学检测技术、化学发光技术、毛细管电泳和免疫学方法。采用紫外/可见分光光度法、荧光和质谱检测的高效液相色谱法是分析 AMK 的最普遍方法。该综述在未来的 AMK 分析方法开发研究领域可能具有重要意义。