Herein, we report a facile, economic, one-pot green synthesis of zinc oxide nanoparticles (ZnO-NPs) for diverse biomedical applications. In the study, ZnO-NPs were synthesized using an aqueous extract of Aquilegia pubiflora as an effective reducing and capping agent. The biomediated nanoparticles were characterized using various techniques including high-performance liquid chromatography (HPLC), X-ray diffraction, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, Dynamic light scattering, photoluminescence, and RAMAN. The particles were highly pure, having an average size of 34.23 nm with spherical or elliptical morphology, and displayed good aqueous dispersion capability. FTIR and HPLC confirmed the successful capping of flavonoids and hydroxycinnamic acid derivatives. The characterized NPs were then explored for their antimicrobial and anti-leishmanial potential. Among the tested bacterial and fungal strains, ZnO-NPs were more potent toward Pseudomonas aeruginosa and Fusarium solani with inhibition zone of 10.3 ± 0.19 mm and 13 ± 1.4 mm, respectively. A dose-dependent cytotoxic effect was observed against Leishmania tropica (KWH23) with significant IC₅₀ for both the promastigote (48 μg/mL) and amastigote form (51 μg/mL) of the parasite. In addition, bacterial kinase enzymes were inhibited by ZnO-NPs, thus allowing us to elaborate a possible action mechanism. Finally, the remarkable biocompatible nature of the particles was confirmed against freshly isolated human red blood cells (hRBCs). Altogether, these results affirmed the high antimicrobial and antiparasitic potential of ZnO-NPs obtained through a biogenic synthetic approach using aqueous extract of the Himalayan Columbine (Aquilegia pubiflora).

在这里，我们报告了一种简便，经济，一锅的绿色合成氧化锌纳米颗粒（ZnO-NPs），用于各种生物医学应用。在这项研究中，ZnO-NPs的合成是使用quil藜作为有效的还原和封端剂。使用各种技术对生物介导的纳米颗粒进行了表征，包括高效液相色谱（HPLC），X射线衍射，傅立叶变换红外光谱（FTIR），扫描电子显微镜，动态光散射，光致发光和RAMAN。该颗粒是高纯度的，具有球形或椭圆形形态的平均尺寸为34.23nm，并且显示出良好的水分散能力。FTIR和HPLC证实成功封盖了类黄酮和羟基肉桂酸衍生物。然后研究表征的NP的抗微生物和抗利什曼原虫潜能。在测试的细菌和真菌菌株中，ZnO-NPs对铜绿假单胞菌 和 枯萎镰刀菌抑制带分别为10.3±0.19 mm和13±1.4 mm。观察到剂量依赖性细胞毒性作用热带利什曼原虫（KWH23）的寄生虫前鞭毛体（48μg/ mL）和鞭毛体形式（51μg/ mL）均具有显着的IC ₅₀。此外，细菌激酶被ZnO-NPs抑制，从而使我们能够阐明可能的作用机制。最终，证实了该颗粒对新鲜分离的人红细胞（hRBC）具有非凡的生物相容性。总之，这些结果证实了通过使用喜马拉雅哥伦拜恩水提取物的生物合成方法获得的ZnO-NP具有很高的抗菌和抗寄生虫潜力（quil藜）。