As ideal tissue engineering scaffold, surface feature plays an important role in cell adhesion and proliferation. In order to improve cytocompatibility of poly(3‐hydroxybutyrate‐co‐hydroxyvalerate) (PHBV) and polyethylene oxide (PEO) synthetic materials, in this study, photochemical surface modification is applied in electrospinning field for the first time. Azidobenzoic acid‐modified gelatin (AZ‐gelatin) as bioactive protein is successfully fixed on PHBV/PEO electrospun mats by photochemical technique without any crosslinking agent. In vitro degradation and cell culture results show that PHBV/PEO/AZ‐gelatin electrospun mats have excellent cell adhesion and proliferation compared with PHBV and PHBV/PEO electrospun mats. But, by contrast with PHBV/PEO electrospun mats, the fiber diameter of PHBV/PEO/AZ‐gelatin mats increased, and the porosity and mechanical properties decrease. Nonetheless, PHBV/PEO/AZ‐gelatin‐0.01 mg mL⁻¹ electrospun mats have shown better morphology (fiber diameter 600 nm, porosity 88%), mechanical properties (4.2 MPa), and water vapor transmission rate (2301 g m⁻² day⁻¹), which has great potential as artificial skin scaffold in tissue regeneration field.

中文翻译：

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具有光化学表面改性的PHBV / PEO / AZ-明胶电纺垫的制备和表征：AZ-明胶的作用

作为理想的组织工程支架，表面特征在细胞粘附和增殖中起着重要作用。为了提高聚（3-羟基丁酸酯-共羟基戊酸酯）（PHBV）和聚环氧乙烷（PEO）合成材料的细胞相容性，本研究首次将光化学表面改性应用于静电纺丝领域。叠氮基苯甲酸改性的明胶（AZ-明胶）作为生物活性蛋白通过光化学技术成功地固定在PHBV / PEO电纺垫上，无需任何交联剂。体外降解和细胞培养结果表明，与PHBV和PHBV / PEO电纺垫相比，PHBV / PEO / AZ-明胶电纺垫具有出色的细胞粘附性和增殖性。但是，与PHBV / PEO电纺垫相比，PHBV / PEO / AZ-明胶垫的纤维直径增加了，并且孔隙率和机械性能降低。尽管如此，PHBV / PEO / AZ-明胶-0.01 mg mL^-1 电纺垫具有更好的形貌（纤维直径600 nm，孔隙率88％），机械性能（4.2 MPa）和水蒸气透过率（2301 gm ^-2 day ^-1），具有作为人造皮肤支架的巨大潜力。组织再生领域。