In this study, an eco-friendly green route strategy was adopted for the synthesis of highly stable, biocompatible polymer-polymer- metal-based composite with high potential anti-bacterial applications. The composite was prepared by modified ionic gelation of Chitosan –gum acacia copolymer with gold nanoparticles (Cs-GA-AuNC). Structural, functional characteristics of the synthesised nanocomposite were studied by spectroscopic, electron microscopic and X-ray diffraction (XRD) studies which reveal functionalised polymers coupled with the gold nanoparticles to form electron core dense nanostructural composite material with notable nanoarchitecture. The synthesised nanocomposite was evaluated for anti-quorum sensing activity against human pathogenic bacterial strain Pseudomonas aeruginosa by measuring the expression of Las R. Nanocomposite treatment brought about drastic reduction of Las R gene expression followed by the remarkable impact on Las R gene-based virulence factors like biofilm formation, pyocyanin production, swarming motility, and total proteolytic activity. Biocompatibility of the composite was tested on Raw cells 246.7 –murine macrophage cell line/The nanocomposite was not inducing any sign of toxicity on the tested Raw cells 246.7 by exhibiting maximum cell viability, lower level of ROS generation and reduced nitride concentration which revealed the noteworthy biocompatibility of synthesised nanocomposite. This selective biological activity shown by the nanocomposite might find its application in the drug delivery for precision medicine.

在这项研究中，采用了一种环保的绿色路线策略来合成具有高潜在抗菌应用的高度稳定，生物相容性聚合物-聚合物-金属基复合材料。复合材料是通过壳聚糖-胶金合欢共聚物与金纳米颗粒（Cs-GA-AuNC）的离子改性凝胶化制备的。通过光谱，电子显微镜和X射线衍射（XRD）研究了合成纳米复合材料的结构，功能特性，揭示了功能化的聚合物与金纳米颗粒结合形成具有显着纳米结构的电子核致密纳米结构复合材料。评价了合成的纳米复合材料对人类致病性细菌铜绿假单胞菌的抗群体感应活性通过测量Las R的表达。纳米复合物处理导致Las R基因表达的急剧降低，随后对基于Las R基因的毒力因子产生了显着影响，如生物膜形成，绿脓素生成，成群运动和总蛋白水解活性。在原始细胞246.7 –鼠巨噬细胞系上测试了复合物的生物相容性/纳米复合物未表现出最大的细胞活力，较低的ROS生成水平和降低的氮化物浓度，因此对所测试的原始细胞246.7不产生任何毒性迹象，这表明值得注意合成纳米复合材料的生物相容性。纳米复合材料显示的这种选择性生物活性可能在精密药物的药物输送中得到应用。