ABC (ATP-binding cassette) proteins can transport metabolic molecules and removes metabolic products and xenobiotics from the cell. The important problem is to study activity and search inhibitors of ABC proteins. There is a problem that ABC-proteins can transport hydrophobic drugs across the cell membrane due to their high substrate specificity. According to published data, Saccharomyces cerevisiae is an ideal model organism for analysis a lot of functional processes and gene activities of human cells. The aim of the present work is to reveal new potential yeast MDR proteins in S. cerevisiae with novel approach based on the cluster analysis.

According to the cluster analysis of yeast ABCB subfamily, STE6 protein is turned out to be the most related to human P-gp protein. The largest number of homologues with human MDR proteins was found in the yeast ABCC subfamily. Yeast BPT1 and YCF1 proteins are shown to be the most phylogenetically close to human MRP1. In the ABCG subfamily of yeast, PDR10, PDR12, PDR15 and PDR18 are turned out to be potential proteins of multidrug resistance. The future experimental study of these subfamilies should be conducted in order to confirm the role of STE6, YCF1, BPT1, PDR10, PDR12, PDR15 and PDR18 in MDR phenotype of yeast and to study their activity modulators.

ABC（ATP结合盒）蛋白可以转运代谢分子，并从细胞中去除代谢产物和异种生物。重要的问题是研究ABC蛋白的活性和搜索抑制剂。由于ABC蛋白的底物特异性高，因此存在可以将疏水性药物跨细胞膜转运的问题。根据公开的数据，酿酒酵母是用于分析人类细胞的许多功能过程和基因活性的理想模型生物。本研究的目的是利用聚类分析的新方法揭示酿酒酵母中新的潜在酵母MDR蛋白。

根据酵母ABCB亚家族的聚类分析，结果证明STE6蛋白与人P-gp蛋白最相关。在酵母ABCC亚家族中发现了与人类MDR蛋白同源的最多的同源物。酵母BPT1和YCF1蛋白显示出与人类MRP1在系统发育上最接近。在酵母的ABCG亚家族中，PDR10，PDR12，PDR15和PDR18被证明是具有多药耐药性的潜在蛋白质。为了确定STE6，YCF1，BPT1，PDR10，PDR12，PDR15和PDR18在酵母MDR表型中的作用并研究其活性调节剂，应该对这些亚家族进行进一步的实验研究。