Highly infectious and obvious withstand ability of Mycobacterium avium subspecies paratuberculosis (MAP) to environment as well as lack of on-site field diagnostic methods notably hampers the paratuberculosis (PTB) control. The existing intricacy, time-consuming and complicated diagnostic methods of PTB accentuate the development of novel and easy-to-perform on-site test. A gold nanoparticle (GNP) based lateral-flow assay (LFA) using MAP recombinant protein (44 kDa) has been developed for sensitive and specific detection of PTB in field conditions. The diagnostic sensitivity and specificity of the LFA for MAP specific antibodies was found approximately 84.2% and 83.3% in comparison to indirect enzyme-linked immunosorbent assay. Consequently, the newly developed GNP based LFA offers on-site and cost-effective method for the prompt diagnosis of PTB and precludes the time-consuming laboratory screening.

极具感染力和明显的承受能力的鸟分枝杆菌亚种副结核（MAP）对环境的影响以及缺乏现场现场诊断方法的情况，尤其会影响肺结核副结核病（PTB）的控制。PTB现有的复杂，耗时且复杂的诊断方法，突显了新颖且易于执行的现场测试的发展。已经开发出使用MAP重组蛋白（44 kDa）的基于金纳米粒子（GNP）的侧向流动测定（LFA），用于在田间条件下灵敏而特异地检测PTB。与间接酶联免疫吸附测定法相比，发现LFA对MAP特异性抗体的诊断敏感性和特异性约为84.2％和83.3％。因此，新开发的基于GNP的LFA提供了一种现场且经济有效的方法来快速诊断PTB，并避免了耗时的实验室筛查。